Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland.
Cells. 2020 May 10;9(5):1185. doi: 10.3390/cells9051185.
The treatment of chronic wounds is still a meaningful challenge to physicians. The aim of this work was to produce vitamin C-enriched chitosan/agarose (CHN/A) film that could serve as potential artificial skin substitute for chronic wound treatment. The biomaterial was fabricated by a newly developed and simplified method via mixing acidic chitosan solution with alkaline agarose solution that allowed to obtain slightly acidic pH (5.97) of the resultant material, which is known to support skin regeneration. Vitamin C was immobilized within the matrix of the film by entrapment method during production process. Produced films (CHN/A and CHN/A + vit C) were subjected to comprehensive evaluation of cellular response with the use of human skin fibroblasts, epidermal keratinocytes, and macrophages. It was demonstrated that novel biomaterials support adhesion and growth of human skin fibroblasts and keratinocytes, have ability to slightly reduce transforming growth factor-beta 1 (TGF-β1) (known to be present at augmented levels in the epidermis of chronic wounds), and increase platelet-derived growth factor-BB (PDGF-BB) secretion by the cells. Nevertheless, addition of vitamin C to the biomaterial formulation does not significantly improve its biological properties due to burst vitamin release profile. Obtained results clearly demonstrated that produced CHN/A film has great potential to be used as cellular dermal, epidermal, or dermo-epidermal graft pre-seeded with human skin cells for chronic wound treatment.
慢性伤口的治疗仍然是医生面临的一个有意义的挑战。本工作的目的是制备富含维生素 C 的壳聚糖/琼脂（CHN/A）膜，作为治疗慢性伤口的潜在人工皮肤替代物。该生物材料是通过混合酸性壳聚糖溶液和碱性琼脂溶液，利用新开发的简化方法制备的，这种方法可以获得略微酸性的 pH 值（5.97），已知这种 pH 值有利于皮肤再生。在生产过程中，通过包埋法将维生素 C 固定在膜基质中。用人类皮肤成纤维细胞、表皮角质形成细胞和巨噬细胞对所制备的薄膜（CHN/A 和 CHN/A+vit C）进行了细胞反应的综合评价。结果表明，新型生物材料支持人皮肤成纤维细胞和角质形成细胞的黏附和生长，具有轻度降低转化生长因子-β1（TGF-β1）（已知在慢性伤口的表皮中存在高水平）的能力，并增加细胞分泌血小板衍生生长因子-BB（PDGF-BB）。然而，由于维生素 C 的突释，将其添加到生物材料配方中并不能显著改善其生物学性能。所得结果清楚地表明，所制备的 CHN/A 薄膜具有很大的潜力，可作为细胞真皮、表皮或真皮-表皮移植物，用于慢性伤口治疗，预先接种了人类皮肤细胞。
