Van Bossuyt H, Desmaretz C, Rombaut B, Wisse E
Laboratory for Cell Biology and Histology, Free University of Brussels, Belgium.
Arch Toxicol. 1988;62(4):316-24. doi: 10.1007/BF00332494.
The effects of Salmonella abortus equi lipopolysaccharides (LPS) on pure cultures of rat Kupffer cells (Kc) were studied. In vitro, LPS is ingested by Kc and located in vacuoles and secondary lysosomes. Culture of Kc in the presence of 1-50 micrograms LPS/ml during 24 h did not affect the viability of the Kc as measured by trypan blue exclusion, neutral red uptake, lactate dehydrogenase leakage and cell survival and spreading. LPS treatment did not influence the ultrastructure of Kc. The exposure of Kc to LPS in vitro did not change the phagocytic activity. Several biochemical processes were stimulated: glucose consumption, MTT-tetrazolium salt reduction, total protein synthesis and secretion of proteins. LPS activated Kc to tumoricidal activity against L929 mouse fibrosarcoma cells. In the light of the above observations, it is concluded that purified LPS is not cytotoxic for pure Kc in culture. On the contrary, LPS stimulates several biochemical and functional processes.
研究了马流产沙门氏菌脂多糖（LPS）对大鼠枯否细胞（Kc）纯培养物的影响。在体外，Kc摄取LPS并将其定位在液泡和次级溶酶体中。在24小时内，在含有1 - 50微克/毫升LPS的条件下培养Kc，通过台盼蓝排斥法、中性红摄取法、乳酸脱氢酶泄漏法以及细胞存活和铺展情况测定，未影响Kc的活力。LPS处理不影响Kc的超微结构。体外将Kc暴露于LPS不会改变其吞噬活性。刺激了几个生化过程：葡萄糖消耗、MTT四氮唑盐还原、总蛋白质合成和蛋白质分泌。LPS激活Kc对L929小鼠纤维肉瘤细胞产生杀肿瘤活性。根据上述观察结果，得出结论：纯化的LPS对培养中的纯Kc无细胞毒性。相反，LPS刺激了几个生化和功能过程。
