Department of Pediatrics Division of Newborn Medicine, Washington University School of Medicine, St. Louis, MO, USA.
Department of Pediatrics, Washington University School of Medicine, St. Louis, MO, USA.
J Matern Fetal Neonatal Med. 2022 May;35(9):1636-1642. doi: 10.1080/14767058.2020.1763298. Epub 2020 May 13.
To determine the prevalence of virus in a previously uncharacterized matched maternal-infant preterm cohort and test if viral presence or viral load correlate with histologic chorioamnionitis, spontaneous preterm labor or pre-eclampsia.
Using qRT-PCR/qPCR we tested plasma or whole blood samples from 56 matched maternal and premature infant dyads for: adenovirus, anellovirus (alphatorquevirus and betatorquevirus), cytomegalovirus (CMV), Epstein-Barr virus (EBV), enterovirus, human herpesvirus 6 (HHV6), parechovirus, and parvovirus B19.
Viral detection was more common in maternal samples 29/56 (52%) than in cord blood from their infants (4/56 (7%)) ( ≤ .0001). No significant difference in viral load or viral prevalence was identified between pregnancies with and without histologic chorioamnionitis, spontaneous preterm labor or pre-eclampsia.
Despite frequent detection of virus in maternal samples, virus was less frequently detected in the infants. Additionally, there was no association of presence or quantity of virus in maternal blood with histologic chorioamnionitis, spontaneous preterm labor or pre-eclampsia in this small, but well-defined cohort. Future studies are necessary to further characterize the role of virus in placental inflammatory states and pregnancy outcomes.
在一个以前未被描述的配对母婴早产队列中确定病毒的流行率，并检验病毒的存在或病毒载量是否与组织学绒毛膜羊膜炎、自发性早产或子痫前期相关。
使用 qRT-PCR/qPCR 法，我们检测了 56 对匹配的母婴早产儿对血浆或全血样本进行了检测，包括腺病毒、圆环病毒（α 和β）、巨细胞病毒（CMV）、EB 病毒（EBV）、肠道病毒、人疱疹病毒 6（HHV6）、小核糖核酸病毒和细小病毒 B19。
病毒检测在母体样本中更为常见（29/56，52%），而在婴儿脐带血中则较少见（4/56，7%）（≤0.0001）。在有或没有组织学绒毛膜羊膜炎、自发性早产或子痫前期的妊娠中，病毒载量或病毒流行率没有显著差异。
尽管在母体样本中频繁检测到病毒，但在婴儿中检测到的病毒较少。此外，在这个小而明确的队列中，母体血液中病毒的存在或数量与组织学绒毛膜羊膜炎、自发性早产或子痫前期之间没有关联。需要进一步的研究来进一步描述病毒在胎盘炎症状态和妊娠结局中的作用。
