Department of Life Sciences, Pachhunga University College, Aizawl, Mizoram, India.
Pharm Biol. 2020 Dec;58(1):393-399. doi: 10.1080/13880209.2020.1760316.
A variety of (L.) R.K. Jansen (Asteraceae) is used by the Mizo people of India and Myanmar for intestinal helminthiasis. To perform a chemical analysis of the plant extract using gas chromatography-mass spectrometry (GC-MS) and test the anthelmintic activity on intestinal parasites. An extract of the aerial parts was prepared in hexane and analysed using GC-MS. Survival test was performed on the cestode, , and the nematode, . Concentrations of 1.25, 2.5, 5, 10 and 20 mg/mL, prepared in phosphate-buffered saline (PBS) with 1% dimethylsulphoxide (DMSO), were tested. Negative control was maintained in PBS with DMSO, and albendazole was used as a reference drug. Each treatment consisted of six worms and was done until death was confirmed. Scanning electron microscopy was used to describe the structural changes. Nineteen compounds were detected. The major compounds were fatty alcohols such as 3,7,11,15-tetramethylhexadec-2-en-1-ol and (9)-9-hexadecen-1-ol. Important bioactive compounds including an alkylamide, -isobutyl-(2,4,8,10)-dodecatetraenamide, and a triterpenoid, lupeol, were also confirmed. The lethal concentration (LC) of the plant extract was 5128.61 ppm on and 8921.50 ppm on . Tegumental shrinkage, erosion of microtriches, and distortion of the suckers were observed on the cestode. The nematode showed collapse of the lips and shrunk cuticle. contains important bioactive compounds, which are responsible for the broad-spectrum anthelmintic activity. Further study on the pharmacology of the compounds is warranted.
印度和缅甸的米佐人使用各种 （L.）R.K. 扬森（菊科）治疗肠道寄生虫病。采用气相色谱-质谱联用（GC-MS）对植物提取物进行化学分析，并对肠道寄生虫进行驱虫活性测试。用己烷制备地上部分提取物，并用 GC-MS 分析。对绦虫 和线虫 进行生存试验。用磷酸盐缓冲盐水（PBS）配制浓度为 1.25、2.5、5、10 和 20 mg/mL 的药物，并用 1%二甲亚砜（DMSO）处理。用 PBS 加 DMSO 作为阴性对照，阿苯达唑作为参考药物。每个处理组由 6 条虫组成，直至确认死亡。用扫描电子显微镜描述结构变化。检测到 19 种化合物。主要化合物为 3,7,11,15-四甲基十六-2-烯-1-醇和（9）-9-十六烯-1-醇等脂肪醇。还确认了包括烷基酰胺、-异丁基-（2,4,8,10）-十二碳四烯酰胺和三萜类化合物羽扇醇在内的重要生物活性化合物。植物提取物对 的致死浓度（LC）为 5128.61 ppm，对 的 LC 为 8921.50 ppm。绦虫的皮层收缩、微毛侵蚀和吸盘变形。线虫的唇塌陷，角质层收缩。该植物含有重要的生物活性化合物，这是广谱驱虫活性的原因。有必要对这些化合物的药理学进行进一步研究。
