Lalthanpuii Pawi Bawitlung, Lalchhandama Kholhring
Department of Life Sciences, Pachhunga University College, Aizawl, Mizoram, India.
Vet World. 2019 Apr;13(4):821-826. doi: 10.14202/vetworld.2020.821-826. Epub 2020 Apr 30.
is used for the treatment of intestinal helminth infections in Mizo traditional medicine. In spite of a variety of drugs developed for helminthiases, an entirely safe and absolutely effective drug is still lacking, so much so that infections remain a major problem in human and animal welfare. In this study, we attempted to substantiate as an anticestodal agent.
The aqueous extract of the aerial parts of was prepared and from it a bioactive fraction was obtained using column chromatography. Chemical analyses were done using thin-layer chromatography (TLC) and gas chromatography-mass spectrometry (GC-MS). Helminth survival test was performed on an intestinal cestode, . Structural effects on the cestode were examined under scanning electron microscopy.
From the bioactive fraction of extract, TLC indicated the presence of an aromatic quinone, which was identified using GC-MS as a quinoline derivative (2,2,4-trimethyl-1,2-dihydroquinoline having a retention time of 24.97 min and chemical formula of CHN). The quinoline-rich fraction showed concentration-dependent activity against as that of albendazole. Scanning electron microscopy of the treated cestode revealed classic anthelmintic effects such as tegumental shrinkage and damage of surface organs. The scolex was shrunk, suckers were degenerated with disintegrated spines, and rostellum was completely collapsed. There were severe damages on the tegument and formation of pit-like scars on the proglottids.
The efficacy of extract and structural damages it caused on the cestode indicates that it is a potential source of anthelmintic agent and that 2,2,4-trimethyl-1,2-dihydroquinoline contributes to its antiparasitic activity.
在米佐传统医学中用于治疗肠道蠕虫感染。尽管已开发出多种用于治疗蠕虫病的药物，但仍缺乏一种完全安全且绝对有效的药物，以至于感染仍是人类和动物健康的一个主要问题。在本研究中，我们试图证实[植物名称]作为一种抗绦虫剂的作用。
制备了[植物名称]地上部分的水提取物，并通过柱色谱从中获得了一个生物活性部分。使用薄层色谱（TLC）和气相色谱 - 质谱联用（GC - MS）进行化学分析。对一种肠道绦虫[绦虫名称]进行蠕虫存活试验。在扫描电子显微镜下检查对绦虫的结构影响。
从[植物名称]提取物的生物活性部分，TLC表明存在一种芳香醌，通过GC - MS鉴定为喹啉衍生物（2,2,4 - 三甲基 - 1,2 - 二氢喹啉，保留时间为24.97分钟，化学式为C₉H₁₃N）。富含喹啉的部分对[绦虫名称]显示出与阿苯达唑相当的浓度依赖性活性。对处理后的绦虫进行扫描电子显微镜检查显示出典型的驱虫效果，如体表收缩和表面器官损伤。头节收缩，吸盘退化且棘突崩解，顶突完全塌陷。节片的体表有严重损伤并形成坑状疤痕。
[植物名称]提取物的功效及其对绦虫造成的结构损伤表明它是驱虫剂的潜在来源，并且2,2,4 - 三甲基 - 1,2 - 二氢喹啉有助于其抗寄生虫活性。
