Laboratório de Neurobiologia Comparada, Departamento de Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brasil.
Graduada em Medicina Veterinária, Fundação Educacional Dom André Arcoverde (UNIFAA), Centro Universitário de Valença, Valença, RJ, Brasil.
Braz J Med Biol Res. 2020;53(6):e9237. doi: 10.1590/1414-431x20209237. Epub 2020 May 8.
We investigated changes in oxidative biomarkers in brain regions such as brainstem, cerebellum, and cerebral cortex of 3-, 6-, 18-, 24-, and 30-month-old rats. We also assessed the effects of low-intensity exercise on these biomarkers in these regions of 6-, 18-, and 24-month-old rats that started exercise on a treadmill at 3, 15, and 21 months of age, respectively. Radiographic images of the femur were taken for all rats. A total of 25 rats (age: twelve 6-, ten 18-, ten 24-, and three 30-month-old rats) were used. Lipid hydroperoxide levels increased in cerebellum at 18 months. Total antioxidant activity exhibited lowest values in brainstem at 3 months. Superoxide dismutase activity did not exhibit significant changes during aging. Total thiol content exhibited lowest values in brain regions of 24- and 30-month-old rats. Exercise reduced total thiol content in brainstem at 6 months, but no change occurred in other regions and other ages. Femur increased its length and width and cortical thickness with advancing age. No change occurred in medullary width. Radiolucency increased and sclerosis was found in cortical and medullary bone with advancing age. Exercise reduced radiolucency and medullary sclerosis. Therefore, aging differentially changed oxidative biomarkers in different brain regions and radiographic measures of the femur. Low-intensity exercise only ameliorated some radiographic measurements of femur. Since the present study possessed limitations (small number of rats per group), a beneficial effect of regular low-intensity exercise on oxidative markers in brain cannot be ruled out.
我们研究了氧化生物标志物在脑区的变化，如脑干、小脑和大脑皮质的 3、6、18、24 和 30 月龄大鼠。我们还评估了低强度运动对这些标志物的影响，这些标志物来自分别于 3、15 和 21 月龄开始在跑步机上运动的 6、18 和 24 月龄大鼠的这些脑区。所有大鼠的股骨都进行了 X 光成像。共有 25 只大鼠（年龄：12 只 6 月龄、10 只 18 月龄、10 只 24 月龄和 3 只 30 月龄）被用于研究。18 月龄时小脑的脂质过氧化物水平增加。3 月龄时，大脑干的总抗氧化活性显示出最低值。超氧化物歧化酶活性在衰老过程中没有显著变化。24 月龄和 30 月龄大鼠的脑区总巯基含量最低。运动降低了 6 月龄大脑干的总巯基含量，但其他脑区和其他年龄没有变化。股骨随着年龄的增长而增加其长度和宽度以及皮质厚度。骨髓宽度没有变化。随着年龄的增长，皮质和骨髓的放射性透明性增加，硬化性增加。运动减少了放射性透明性和骨髓硬化。因此，衰老以不同的方式改变了不同脑区的氧化生物标志物和股骨的 X 光测量值。低强度运动仅改善了股骨的一些 X 光测量值。由于本研究存在局限性（每组大鼠数量较少），因此不能排除定期低强度运动对大脑氧化标志物的有益影响。
