Sadalage Priyadarshani S, Patil Reshma V, Padvi Mukesh N, Pawar Kiran D
School of Nanoscience and Biotechnology, Shivaji University, Kolhapur, Maharashtra, India.
School of Nanoscience and Biotechnology, Shivaji University, Kolhapur, Maharashtra, India.
Colloids Surf B Biointerfaces. 2020 Sep;193:111084. doi: 10.1016/j.colsurfb.2020.111084. Epub 2020 Apr 28.
The safety of drinking water is one of the most important public health issues as very high concentrations of metal like iron acts as a useful surrogate for other heavy metals. The present study demonstrates the use of almond skin extract (ASE) for simple and rapid synthesis of antibacterial silver nanoparticles (AgNPs) for the development of a highly selective and sensitive colorimetric method for the detection of Fe in water samples. The optimization of various biogenic synthesis parameters showed ASE:AgNO ratio of 4:1,1 mM of AgNO, pH 6 and incubation for 10 min at 70 °C were the optimum conditions. The test of antibacterial activity against widely used, representative Gram-negative and positive bacteria showed that AgNPs exhibit good activity against all five tested bacterial strains and comparatively were more effective against Gram-negative bacteria. Further, the test of AgNPs as a colorimetric probe for the detection of 20 different metal ions demonstrated that AgNPs were highly selective and sensitive towards the detection of Fe. The study of sensitivity of Fe detection showed 245 ppm as the Limit of detection whereas, the intra-day recovery of Fe in the range of 87.2-100.1 % with %RSD in the range of 4.2-6.5 % and inter-day recovery of Fe in the range of 92.02-96.59 % with %RSD in the range of 2.9-3.8 % demonstrated the excellent precision and accuracy of the assay method. Thus, our AgNPs based selective and sensitive assay can be applied to the analysis of iron in drinking water samples.
饮用水安全是最重要的公共卫生问题之一，因为高浓度的铁等金属可作为其他重金属的有效替代指标。本研究展示了利用杏仁皮提取物（ASE）简单快速地合成抗菌银纳米颗粒（AgNPs），以开发一种用于检测水样中铁的高选择性和灵敏的比色法。各种生物合成参数的优化表明，ASE与硝酸银（AgNO₃）的比例为4:1、硝酸银浓度为1 mM、pH值为6以及在70℃下孵育10分钟是最佳条件。针对广泛使用的代表性革兰氏阴性菌和阳性菌的抗菌活性测试表明，AgNPs对所有五种测试菌株均表现出良好的活性，且相对而言对革兰氏阴性菌更有效。此外，将AgNPs作为比色探针检测20种不同金属离子的测试表明，AgNPs对铁的检测具有高度选择性和灵敏性。铁检测灵敏度的研究表明，检测限为245 ppm，而铁的日内回收率在87.2 - 100.1%范围内，相对标准偏差（%RSD）在4.2 - 6.5%范围内，日间回收率在92.02 - 96.59%范围内，%RSD在2.9 - 3.8%范围内，这证明了该检测方法具有出色的精密度和准确性。因此，我们基于AgNPs的选择性和灵敏检测方法可应用于饮用水样中铁的分析。
