捷克共和国两例儿童中分离的 G9P[4]轮状病毒 A 重组株全基因组序列。
Whole-genome sequence of a reassortant G9P[4] rotavirus A strain from two children in the Czech Republic.
机构信息
Veterinary Research Institute, Brno, Czech Republic.
Department of Microbiology, University Hospital Olomouc and Faculty of Medicine, Palacký University, Olomouc, Czech Republic.
出版信息
Arch Virol. 2020 Jul;165(7):1703-1706. doi: 10.1007/s00705-020-04648-w. Epub 2020 May 13.
An unusual reassortant rotavirus A (RVA) strain was isolated during RVA surveillance in two previously hospitalized children in 2018. G/P typing revealed uncommon G9P[4] genotypes, so the strains were further characterized by Illumina next-generation sequencing. Whole-genome typing showed that the two strains had a DS-1-like backbone except for NSP2: G9-P[4]-I2-R2-C2-M2-A2-N1-T2-E2-H2. The two strains shared 99.9-100% nucleotide sequence identity in all genes.
在 2018 年对两名既往住院患儿进行轮状病毒 A（RVA）监测时，分离到一株罕见的重组 RVA 毒株。G/P 型分析显示，该毒株为不常见的 G9P[4]基因型，因此进一步采用 Illumina 新一代测序技术对其进行特征分析。全基因组型分析显示，两株病毒均具有 DS-1 样的基因组长，除 NSP2 区外，其 G9-P[4]-I2-R2-C2-M2-A2-N1-T2-E2-H2。两株病毒在所有基因中均具有 99.9%-100%的核苷酸序列同一性。
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