Future Industries Institute, School of Engineering, University of South Australia, Mawson Lakes, Adelaide, SA, 5095, Australia.
Global Innovative Centre for Advanced Nanomaterials, Faculty of Engineering and Built Environment, The University of Newcastle, Callaghan, NSW, 2308, Australia.
Small. 2020 Jun;16(25):e2000285. doi: 10.1002/smll.202000285. Epub 2020 May 14.
Nanoparticles have become an important utility in many areas of medical treatment such as targeted drug and treatment delivery as well as imaging and diagnostics. These advances require a complete understanding of nanoparticles' fate once placed in the body. Upon exposure to blood, proteins adsorb onto the nanoparticles surface and form a protein corona, which determines the particles' biological fate. This study reports on the protein corona formation from blood serum and plasma on spherical and rod-shaped nanoparticles. These two types of mesoporous silica nanoparticles have identical chemistry, porosity, surface potential, and size in the y-dimension, one being a sphere and the other a rod shape. The results show a significantly larger amount of protein attaching from both plasma and serum on the rod-like particles compared to the spheres. Interrogation of the protein corona by liquid chromatography-mass spectrometry reveals shape-dependent differences in the adsorption of immunoglobulins and albumin proteins from both plasma and serum. This study points to the need for taking nanoparticle shape into consideration because it can have a significant impact on the fate and therapeutic potential of nanoparticles when placed in the body.
纳米颗粒在医学治疗的许多领域已经成为一种重要的工具，如靶向药物和治疗药物的输送以及成像和诊断。这些进展需要完全了解纳米颗粒一旦进入体内的命运。纳米颗粒暴露于血液中时，蛋白质会吸附在纳米颗粒表面并形成蛋白质冠，从而决定颗粒的生物学命运。本研究报告了球形和棒状纳米颗粒在血清和血浆中的蛋白质冠形成情况。这两种类型的介孔硅纳米颗粒具有相同的化学性质、孔隙率、表面电位和 y 维度的尺寸，一种是球形，另一种是棒状。结果表明，与球形相比，棒状颗粒从血浆和血清中吸附的蛋白质要多得多。通过液相色谱-质谱法对蛋白质冠进行询问，揭示了来自血浆和血清的免疫球蛋白和白蛋白蛋白质吸附的形状依赖性差异。本研究表明，需要考虑纳米颗粒的形状，因为当将纳米颗粒放入体内时，它会对纳米颗粒的命运和治疗潜力产生重大影响。
