School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China; Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050, China.
Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing 100050, China.
Int J Biol Macromol. 2020 Sep 15;159:129-136. doi: 10.1016/j.ijbiomac.2020.05.050. Epub 2020 May 12.
Lentinan is widely used as a therapeutic agent for treatment of malignant tumors in clinical practice. The chemical structure of lentinan is highly associated with its biological activity. In this study, the correlation between the structure of lentinan and its immune activity was investigated to assess the function of key parameters that can influence quality control of lentinan. The results showed that the batch-to-batch consistency of two lentinan samples was satisfactory, indicating the stability of production process of lentinan. However, although the chemical composition and triple-helical conformation (THC) of the tested samples were relatively similar, their Mw, polydispersity index (PDI), and Rg remarkably varied due to different production processes. In vitro immunomodulatory assay reflects that lentinan could stimulate the macrophages phagocytic capacity. Meanwhile, lentinan samples could improve the spleen and thymus indices, promote the proliferation of lymphocytes and adjust for the percentages of CD4+ and CD8+ T cells in vivo. Furthermore, the immunomodulatory effect of lentinan sample B (Mw: 650,700 g/mol) was superior than that of the sample A (Mw: 4,818,700 g/mol). It was noted that the Mw should be detected as a necessary index for quality control of lentinan to ensure stability and effectiveness of the production process.
香菇多糖在临床上被广泛用作治疗恶性肿瘤的治疗剂。香菇多糖的化学结构与其生物活性密切相关。在这项研究中，研究了香菇多糖的结构与其免疫活性之间的相关性，以评估影响香菇多糖质量控制的关键参数的功能。结果表明，两个香菇多糖样品的批间一致性令人满意，表明香菇多糖生产过程的稳定性。然而，尽管测试样品的化学成分和三螺旋构象（THC）相对相似，但由于生产工艺不同，它们的Mw、多分散指数（PDI）和 Rg 显著不同。体外免疫调节试验表明，香菇多糖可刺激巨噬细胞的吞噬能力。同时，香菇多糖样品可提高脾和胸腺指数，促进淋巴细胞增殖，并调节体内 CD4+和 CD8+T 细胞的百分比。此外，香菇多糖样品 B（Mw：650,700 g/mol）的免疫调节作用优于样品 A（Mw：4,818,700 g/mol）。值得注意的是，Mw 应作为香菇多糖质量控制的必要指标进行检测，以确保生产过程的稳定性和有效性。
