Key Lab for Agro-product Processing and Quality Control of Nanchang City, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Int J Biol Macromol. 2020 Sep 15;159:108-116. doi: 10.1016/j.ijbiomac.2020.04.265. Epub 2020 May 12.
In this study, a crude and purified polysaccharide from Cyclocarya paliurus (CPP, CPP) were performed with chlorosulfonic acid-pyridine (CSA-Pyr) method to obtain sulfated derivatives (S-CPP, S-CPP). After comparatively investigating, characterization results showed that the modifications were successful. Polysaccharides were used to culture mouse bone marrow-derived dendritic cells (BM-DCs) to evaluate their immunomodulatory activity and explore mechanism. The functional activity of CPP was significantly stronger than that of the purified polysaccharide CPP. Meanwhile, S-CPP showed stronger immunomodulatory activity than CPP through determination of cytokine expression levels. We found that p-JNK, p-p38MAPK and NF-κB p65 proteins were significantly increased by stimulus of CPP and S-CPP, blocking TLR2/4 could significantly decreased proteins above which proved that immune regulation effect of CPP and S-CPP on DCs was performed via MAPK and NF-κB signaling pathways by triggering TLR2/4. S-CPP could serve as potential immunomodulatory agents used as complementary medicine or functional foods.
在这项研究中，采用氯磺酸-吡啶（CSA-Pyr）法对青钱柳叶粗多糖（CPP）和纯化多糖（CPP）进行了修饰，得到了硫酸化衍生物（S-CPP、S-CPP）。经过比较研究，表征结果表明修饰成功。多糖被用于培养小鼠骨髓来源的树突状细胞（BM-DC），以评估其免疫调节活性并探索其机制。CPP 的功能活性明显强于纯化多糖 CPP。同时，通过细胞因子表达水平的测定，S-CPP 表现出比 CPP 更强的免疫调节活性。我们发现 CPP 和 S-CPP 刺激后 p-JNK、p-p38MAPK 和 NF-κB p65 蛋白显著增加，阻断 TLR2/4 后可显著降低上述蛋白，证明 CPP 和 S-CPP 通过触发 TLR2/4 对 DC 的免疫调节作用是通过 MAPK 和 NF-κB 信号通路实现的。S-CPP 可作为潜在的免疫调节剂，用于补充医学或功能性食品。
