Department of Prosthodontics, School of Oral Health Science, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa.
Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2193, South Africa.
Int J Environ Res Public Health. 2020 May 12;17(10):3345. doi: 10.3390/ijerph17103345.
: Contamination with oral commensals and pathogenic microorganisms, and cross contamination between clinic and laboratory can occur. The amount of contamination has not been determined. : Samples from different clinical and laboratory stages before and after disinfection (17 sites, 10 samples per stage) were collected. Laboratory surfaces and equipment were swabbed for 10 days (11 sites). Swabs were cultured for total mixed flora, Streptococci, Lactobacilli, , aerobic Gram-negative bacteria (AGNB) and . Knowledge of infection control among staff and students was assessed. : Clinic: In total, 30-40% of the samples overall were contaminated with mixed flora and Streptococci of >100 cfu/swab; >100 cfu of AGNB and were present on 6% and 1% of samples; 2% contained <100 cfu of . Laboratory: In total, 17-48% of the samples overall were contaminated with mixed flora and Streptococci of >100 cfu/swab; >100 cfu of AGNB were present on 11% of samples; none contained >100 cfu of . Disinfection significantly reduced the level of all organisms. Knowledge of infection control was sufficient, but compliance was poor. : Although the count of mixed flora was high, potential pathogens such as and were low. In immunocompromised patients, this can become a problem.
: 可能会发生口腔共生菌和病原菌的污染,以及临床和实验室之间的交叉污染。污染的程度尚未确定。: 在消毒前后的不同临床和实验室阶段(17 个部位,每个阶段 10 个样本)采集样本。实验室表面和设备连续 10 天进行拭子采样(11 个部位)。对总混合菌群、链球菌、乳杆菌、需氧革兰氏阴性菌(AGNB)和进行拭子培养。评估工作人员和学生的感染控制知识。: 临床:总共,30-40%的样本整体混合菌群和>100 cfu/拭子的链球菌污染;>100 cfu/拭子的 AGNB 和存在于 6%和 1%的样本;2%的样本中含有<100 cfu/。实验室:总共,17-48%的样本整体混合菌群和>100 cfu/拭子的链球菌污染;>100 cfu/拭子的 AGNB 存在于 11%的样本;没有样本含有>100 cfu/。消毒显著降低了所有生物的水平。感染控制知识充足,但合规性差。: 尽管混合菌群的数量很高,但潜在的病原体如和很少。在免疫功能低下的患者中,这可能会成为一个问题。