Mothibe J V, Patel M
Division of Oral Microbiology, Department of Oral Biological Sciences, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Private Bag 3, Wits, 2050 Johannesburg, South Africa.
Division of Oral Microbiology, Department of Oral Biological Sciences, School of Oral Health Sciences, Faculty of Health Sciences, University of the Witwatersrand, Private Bag 3, Wits, 2050 Johannesburg, South Africa.
Microb Pathog. 2017 Sep;110:128-134. doi: 10.1016/j.micpath.2017.06.036. Epub 2017 Jun 24.
Candida albicans cause opportunistic infections including oral candidiasis in immunocompromised patients. It has an ability to cause infection due to its virulence factors. This study investigated the pathogenic characteristics of C. albicans isolated from the oral cavities of healthy subjects and two vulnerable groups, denture wearers and cancer patients wearing oral prostheses. Oral rinse samples were collected and cultured for the quantitative and qualitative analysis of Candida. Twenty strains of C. albicans isolated from the healthy individuals and denture wearers and, 14 strains isolated from the cancer patients were selected and their pathogenic characteristics were measured. The results of the study groups were compared using a Scheffe test for pairwise comparison and a chi square test. Denture wearer and cancer patients with prostheses carried significantly higher number (p < 0.01) and a variety of Candida than the normal individuals. Denture wearer and cancer patients carried several Candida species. The adherence abilities (p = 0.01) as well as phospholipase (p < 0.01) and proteinase (p = 0.03) production were significantly higher in the strains from denture wearers. In addition, high number of isolates from the denture wearers produced phospholipase and proteinase (85% and 80% respectively) compared to the strains from normal subjects (25% and 60% respectively). Only the germ tube formation and adherence ability were significantly higher in the strains from the cancer patients with prostheses (p = 0.05 and p < 0.01 respectively). In conclusion, during the commensal state, the increased expression of virulence factors in the denture wearers suggests the readiness of these strains to cause infection in this group. The high number of C. albicans and their increased adherence ability in the two study groups suggest that hygiene of oral cavity and prostheses is important in the prevention of colonization of Candida and the development of oral candidiasis.
白色念珠菌可引发机会性感染，包括免疫功能低下患者的口腔念珠菌病。由于其毒力因子，它具有引发感染的能力。本研究调查了从健康受试者以及两个易感群体（佩戴假牙者和佩戴口腔假体的癌症患者）口腔中分离出的白色念珠菌的致病特征。收集口腔冲洗样本并进行培养，以对念珠菌进行定量和定性分析。从健康个体和佩戴假牙者中分离出20株白色念珠菌，从癌症患者中分离出14株白色念珠菌，并对它们的致病特征进行测定。使用谢费检验进行两两比较，并采用卡方检验对研究组的结果进行比较。佩戴假牙者和佩戴假体的癌症患者携带的念珠菌数量显著更高（p < 0.01），且种类更多。佩戴假牙者携带多种念珠菌。佩戴假牙者菌株的黏附能力（p = 0.01）以及磷脂酶（p < 0.01）和蛋白酶（p = 0.03）的产生显著更高。此外，与正常受试者的菌株相比，佩戴假牙者分离出的大量菌株产生磷脂酶和蛋白酶（分别为85%和80%）（正常受试者分别为25%和60%）。只有佩戴假体的癌症患者菌株的芽管形成和黏附能力显著更高（分别为p = 0.05和p < 0.01）。总之，在共生状态下，佩戴假牙者毒力因子表达的增加表明这些菌株易于在该群体中引发感染。两个研究组中白色念珠菌数量众多且黏附能力增强，这表明口腔和假体的卫生对于预防念珠菌定植和口腔念珠菌病的发生很重要。
