Moon E, Wu R
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.
Gene. 1988 Oct 15;70(1):1-12. doi: 10.1016/0378-1119(88)90099-6.
We describe the isolation and organization, at the nucleotide sequence level, of genes located at the two junctions of the large single-copy region (LSCR) and the two inverted repeats (IRA and IRB) in the rice chloroplast genome. This is the first example where the two junctions are precisely located in a monocot. In rice, a ribosomal protein gene cluster, rpl23-rpl2-rps19, which codes for the ribosomal proteins L23 (rpl23), L2 (rpl2) and S19 (rps19), lies at the ends of the two IRs near the LSCR. The inverted repeats end 45 bp from the translation stop codon of rps19. The gene for the 32-kDa photosystem II protein, psbA, is located at the extremity of the LSCR near IRA, and transcribed towards IRA. The translation stop codon of psbA is 68 bp from the right-hand junction (JLA). Thus, JLA is located within the intergenic sequence of the two genes, rps19 and psbA. Around the left-hand junction (JLB), there is a typical ribosomal protein gene cluster, rpl23-rpl2-rps19-rpl22 (rpl22 for the ribosomal protein L22). The translation start codon of rpl22 is located in the LSCR 25 bp from JLB. Therefore, JLB is located within the intergenic sequence between rps19 and rpl22.
我们描述了水稻叶绿体基因组中位于大单拷贝区域（LSCR）与两个反向重复序列（IRA和IRB）的两个连接处的基因在核苷酸序列水平上的分离与组织情况。这是首次在单子叶植物中精确确定两个连接处的位置。在水稻中，一个编码核糖体蛋白L23（rpl23）、L2（rpl2）和S19（rps19）的核糖体蛋白基因簇rpl23 - rpl2 - rps19位于靠近LSCR的两个IR末端。反向重复序列在rps19的翻译终止密码子下游45 bp处结束。32 kDa光系统II蛋白的基因psbA位于靠近IRA的LSCR末端，并朝着IRA转录。psbA的翻译终止密码子距离右手连接处（JLA）68 bp。因此，JLA位于rps19和psbA这两个基因的基因间序列内。在左手连接处（JLB）周围，有一个典型的核糖体蛋白基因簇rpl23 - rpl2 - rps19 - rpl22（rpl22编码核糖体蛋白L22）。rpl22的翻译起始密码子位于距离JLB 25 bp的LSCR中。因此，JLB位于rps19和rpl22之间的基因间序列内。
