UNESCO UNISA Africa Chair in Nanosciences and Nanotechnology, College of Graduate Studies, University of South Africa (UNISA), Pretoria, South Africa; NANOAFNET (Nanosciences African Network), Materials Research Department, iThemba LABS, Cape Town, South Africa.
Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110890. doi: 10.1016/j.msec.2020.110890. Epub 2020 Mar 21.
Maghemite (FeO-NPs) nanoparticles were synthesized by a convenient, green and cost effective method using aqueous fruit extracts of Hyphaene thebaica. Different techniques like FTIR, XRD, UV-Vis, Raman, HR-TEM, EDS. SAED, Zeta potential were used to establish the nature of FeO-NPs, while the therapeutic properties were studied using different biological assays including antiviral, antibacterial, antifungal, antioxidant and enzyme inhibition assays. XRD pattern revealed sharp peaks and a crystalline nature of FeO-NPs. HR-TEM revealed quasi-spherical and cuboidal morphologies, while the particle size in ~10 nm. FTIR indicated a sharp peak centered at ~444 cm which is the characteristic FeO band vibration. SAED pattern indicated the crystalline nature while EDS also confirmed the synthesis of FeO NPs. Zeta potential was obtained in different solvents and physiological buffers indicating highest value in water (-26.5 mV) and lowest in DMSO (-15.8 mV). Tested bacterial strains, Bacillus subtilis was found to be inhibited significantly. Aspergillus flavus appeared to be susceptible to all of the tested concentration of FeO NPs. Maximum 40.78% FRSA was obtained at 400 μg/mL. Cell culture based studies on RD cells and L20B cells indicated reduction in viability of cells with increase concentration of FeO NPs. Moderate inhibition of polio virus-1 and polio virus-2 was observed, after culturing the virus in the L20B cells. Excellent Protein Kinase (PK) inhibition was revealed. Hemolytic potential and cytotoxic potential was indicated to be dose dependent. In conclusion, the present report for the first time reports the synthesis of FeO NPs from H. thebaica fruits and reveals their biomedical potential including antiviral potential.
磁赤铁矿（FeO-NPs）纳米颗粒通过一种简便、绿色且经济高效的方法合成，使用的是 Hyphaene thebaica 的水提水果提取物。不同的技术，如 FTIR、XRD、UV-Vis、Raman、HR-TEM、EDS、SAED 和 Zeta 电位，用于确定 FeO-NPs 的性质，而治疗特性则通过不同的生物测定进行研究，包括抗病毒、抗菌、抗真菌、抗氧化和酶抑制测定。XRD 图谱显示出尖锐的峰和 FeO-NPs 的结晶性质。HR-TEM 显示出类球形和立方体形貌，而粒径约为 10nm。FTIR 表明中心在约 444cm 处有一个尖锐的峰，这是 FeO 带振动的特征峰。SAED 图案表明结晶性质，EDS 也证实了 FeO NPs 的合成。在不同的溶剂和生理缓冲液中获得了 Zeta 电位，表明在水中（-26.5mV）的数值最高，在 DMSO 中（-15.8mV）的数值最低。测试的细菌菌株，枯草芽孢杆菌被发现受到显著抑制。黄曲霉似乎对所有测试浓度的 FeO-NPs 都敏感。在 400μg/mL 时获得最大的 40.78%FRSA。基于 RD 细胞和 L20B 细胞的细胞培养研究表明，随着 FeO-NPs 浓度的增加，细胞活力降低。在 L20B 细胞中培养病毒后，观察到对脊髓灰质炎病毒-1 和脊髓灰质炎病毒-2 的中度抑制。揭示了优异的蛋白激酶（PK）抑制作用。溶血和细胞毒性作用呈剂量依赖性。总之，本报告首次报道了从 H. thebaica 果实中合成 FeO-NPs，并揭示了它们的生物医学潜力，包括抗病毒潜力。
