Department of Microbiology & Molecular Genetics, Faculty of Life Sciences, The Women University, Multan 66000, Pakistan.
Wet Chemistry Laboratory, Department of Metallurgical Engineering, NED University of Engineering and Technology, University Road, Karachi 75270, Pakistan.
Molecules. 2023 Sep 7;28(18):6504. doi: 10.3390/molecules28186504.
Green approaches for nanoparticle synthesis have emerged as biocompatible, economical, and environment-friendly alternatives to counteract the menace of microbial drug resistance. Recently, the utilization of honey as a green source to synthesize FeO-NPs has been introduced, but its antibacterial activity against one of the opportunistic MDR pathogens, , has not been explored. Therefore, this study employed honey as a reducing and capping agent for the synthesis of iron oxide nanoparticles (FeO-NPs). Subsequent to the characterization of nanoparticles, their antibacterial, antioxidant, and anti-inflammatory properties were appraised. In UV-Vis spectroscopic analysis, the absorption band ascribed to the SPR peak was observed at 350 nm. XRD analysis confirmed the crystalline nature of FeO-NPs, and the crystal size was deduced to be 36.2 nm. Elemental analysis by EDX validated the presence of iron coupled with oxygen in the nanoparticle composition. In ICP-MS, the highest concentration was of iron (87.15 ppm), followed by sodium (1.49 ppm) and other trace elements (<1 ppm). VSM analysis revealed weak magnetic properties of FeO-NPs. Morphological properties of FeO-NPs revealed by SEM demonstrated that their average size range was 100-150 nm with a non-uniform spherical shape. The antibacterial activity of FeO-NPs was ascertained against 30 clinical isolates of , with the largest inhibition zone recorded being 10 mm. The MIC value for FeO-NPs was 30 µg/mL. However, when mingled with three selected antibiotics, FeO-NPs did not affect any antibacterial activity. Momentous antioxidant (IC = 22 µg/mL) and anti-inflammatory (IC = 70 µg/mL) activities of FeO-NPs were discerned in comparison with the standard at various concentrations. Consequently, honey-mediated FeO-NP synthesis may serve as a substitute for orthodox antimicrobial drugs and may be explored for prospective biomedical applications.
绿色的纳米粒子合成方法已经成为对抗微生物药物耐药性威胁的生物相容性、经济实惠和环境友好的替代方法。最近，利用蜂蜜作为绿色来源来合成 FeO-NPs 的方法已经被提出，但是它对一种机会性病原体 MDR 的抗菌活性还没有被探索。因此，本研究使用蜂蜜作为还原剂和稳定剂来合成氧化铁纳米粒子（FeO-NPs）。在对纳米粒子进行表征之后，评估了它们的抗菌、抗氧化和抗炎特性。在紫外可见光谱分析中，观察到归因于 SPR 峰的吸收带在 350nm 处。XRD 分析证实了 FeO-NPs 的结晶性质，并且推断出晶体尺寸为 36.2nm。EDX 元素分析验证了纳米粒子组成中铁与氧的存在。在 ICP-MS 中，最高浓度是铁（87.15ppm），其次是钠（1.49ppm）和其他痕量元素（<1ppm）。VSM 分析显示 FeO-NPs 具有较弱的磁性。SEM 显示的 FeO-NPs 形态特性表明，它们的平均尺寸范围为 100-150nm，具有非均匀的球形。FeO-NPs 的抗菌活性针对 30 株临床分离株的 进行了确定，记录的最大抑制圈为 10mm。FeO-NPs 的 MIC 值为 30µg/mL。然而，当与三种选定的抗生素混合时，FeO-NPs 不会影响任何抗菌活性。在各种浓度下，与标准相比，FeO-NPs 具有显著的抗氧化（IC=22µg/mL）和抗炎（IC=70µg/mL）活性。因此，蜂蜜介导的 FeO-NP 合成可以作为传统抗菌药物的替代品，并可能被探索用于未来的生物医学应用。
