Bello Bello Aminu, Khan Shahid Ali, Khan Jalaluddin Awllia, Syed Fareeduddin Quadri, Mirza Muqtadir Baig, Shah Luqman, Khan Sher Bahadar
Department of Biochemistry, King Abdul-Aziz University, Jeddah, Saudi Arabia; Department of Biochemistry, Federal University Dutse, P.M.B.7156, Dutse, Jigawa State, Nigeria.
Center of Excellence for Advanced Materials Research (CEAMR), King Abdul-Aziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.
Biochem Biophys Res Commun. 2017 Aug 26;490(3):889-894. doi: 10.1016/j.bbrc.2017.06.136. Epub 2017 Jun 22.
We present here the biosynthesis of AgNps from the aqueous extract of H. thebaica fruit, and monitored through UV-Vis spectrophotometer. The functional group were characterized through ATR-FTIR spectroscopy, the particle size, morphologies and elemental composition of the nanoparticles were investigated by using TEM, FESEM and EDS respectively. The anti-proliferation activity of the synthesized AgNps was carried out using MTT assay on human prostate (PC3), breast (MCF7) and liver (HepG2) cancer cell lines. The anti-proliferation assay showed that the AgNps were able to inhibit the proliferation of the cancer cell lines in a dose depending manner. The effect was found more pronounced on prostate (IC 2.6 mg/mL) followed by breast (IC 4.8 mg/mL) and then liver cancer cell lines (IC 6.8 mg/mL). The prepared AgNps were found to inhibit 99% growth of both E. coli and S. aureus after 24 h of incubation. The nanoparticles were used for the degradation of 4-nitrophenol (4-NP) and Congo red dyes (CR), which efficiently degrade CR, but make complex formation with 4-NP. Therefore, the AgNps synthesized from the aqueous fruit extract of H. thebaica have potential application in pharmacology and waste water treatment.
我们在此展示了从埃及天仙子果实水提取物中生物合成银纳米颗粒，并通过紫外可见分光光度计进行监测。通过衰减全反射傅里叶变换红外光谱对官能团进行了表征，分别使用透射电子显微镜（TEM）、场发射扫描电子显微镜（FESEM）和能谱仪（EDS）研究了纳米颗粒的粒径、形态和元素组成。使用MTT法对人前列腺癌（PC3）、乳腺癌（MCF7）和肝癌（HepG2）细胞系进行了合成银纳米颗粒的抗增殖活性检测。抗增殖检测表明，银纳米颗粒能够以剂量依赖的方式抑制癌细胞系的增殖。发现对前列腺癌细胞系（IC50为2.6mg/mL）的作用最为明显，其次是乳腺癌细胞系（IC50为4.8mg/mL），然后是肝癌细胞系（IC50为6.8mg/mL）。在孵育24小时后，制备的银纳米颗粒被发现可抑制大肠杆菌和金黄色葡萄球菌99%的生长。这些纳米颗粒用于降解4-硝基苯酚（4-NP）和刚果红染料（CR），它们能有效降解CR，但会与4-NP形成络合物。因此，从埃及天仙子果实水提取物中合成的银纳米颗粒在药理学和废水处理方面具有潜在应用价值。
