Mamidi Narsimha, Delgadillo Ramiro Manuel Velasco, González-Ortiz Aldo
Tecnologico de Monterrey, Department of Chemistry and Nanotechnology, School of Engineering and Sciences, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico.
Tecnologico de Monterrey, Department of Chemistry and Nanotechnology, School of Engineering and Sciences, Ave. Eugenio Garza Sada 2501, Monterrey 64849, NL, Mexico.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111698. doi: 10.1016/j.msec.2020.111698. Epub 2020 Nov 4.
Engineered stimuli-responsive drug delivery strategies grasp enormous potential in biomedical applications for disease treatment due to their exploited therapeutic efficiency. In the current study, we developed poly 4-hydroxyphenyl methacrylate-carbon nano-onions (PHPMA-CNOs = f-CNOs) embedded bovine serum albumin (BSA) nanocomposite fibers by Forcespinning® (FS) technology for stimuli-responsive release of cargo, using doxorubicin (DOX) as a model drug. Nanocomposite fiber system showed thermosensitive drug release and exhibited around 72 and 95% of drug release at 37 and 43 °C, respectively. A slow and prolonged DOX release was observed over a 15-day study. The amount of drug released was determined by the concentration of the DOX payload, incubation temperature, and pH of the released medium. Owing to the f-CNOs incorporation, the mechanical strength (18.23 MPa) of hybrid BSA nanocomposite fibers was enhanced significantly. Besides, in vitro degradation, water contact angles, and thermal properties of nanocomposite fibers have augmented. During the in vitro cytotoxicity assessment, nanocomposite fibers exhibited improved cell viability against human fibroblast cells. Nonetheless, the external-stimuli-dependent and sustained DOX release perhaps reduces its circumventing side effects and show potential applications in biomedical research.
由于其显著的治疗效果，工程化的刺激响应药物递送策略在生物医学疾病治疗应用中具有巨大潜力。在本研究中，我们通过强力旋转（Forcespinning®，FS）技术开发了聚甲基丙烯酸4-羟苯酯-碳纳米洋葱（PHPMA-CNOs = f-CNOs）包埋牛血清白蛋白（BSA）的纳米复合纤维，用于刺激响应式货物释放，以阿霉素（DOX）作为模型药物。纳米复合纤维系统呈现出热敏性药物释放，在37℃和43℃时分别有大约72%和95%的药物释放。在为期15天的研究中观察到阿霉素的缓慢且持续的释放。释放的药物量由阿霉素负载浓度、孵育温度和释放介质的pH值决定。由于掺入了f-CNOs，混合BSA纳米复合纤维的机械强度（18.23MPa）显著提高。此外，纳米复合纤维的体外降解、水接触角和热性能也有所增强。在体外细胞毒性评估中，纳米复合纤维对人成纤维细胞显示出改善的细胞活力。尽管如此，外部刺激依赖且持续的阿霉素释放可能会减少其规避副作用的能力，并在生物医学研究中显示出潜在应用。
