Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, CA, USA.
Methods Mol Biol. 2020;2144:47-56. doi: 10.1007/978-1-0716-0592-9_4.
The laboratory fruit fly Drosophila melanogaster is one of the leading models for the study of aging. Whereas several behavioral and physiological biomarkers of aging have been identified for Drosophila, lifespan remains the most robust measure of aging rate. Aging and lifespan can be modulated by genetic alterations, as well as by drugs and dietary components, to reveal basic and conserved mechanisms of aging. Here methods are presented for Drosophila lifespan assay, including media preparation, supplementation of media with various drugs, culturing of the flies, passaging flies and recording deaths, and the analysis of lifespan data.
实验室果蝇 Drosophila melanogaster 是衰老研究的主要模式生物之一。虽然已经确定了几种用于果蝇的衰老行为和生理生物标志物,但寿命仍然是衡量衰老速度的最可靠指标。衰老和寿命可以通过遗传改变以及药物和饮食成分来调节,以揭示衰老的基本和保守机制。本文介绍了用于果蝇寿命测定的方法,包括培养基的制备、用各种药物补充培养基、培养果蝇、传代和记录死亡以及寿命数据的分析。