Institute of Animal Genetics and Breeding, Sichuan Agricultural University, Chengdu, China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China.
PLoS One. 2020 Oct 16;15(10):e0240596. doi: 10.1371/journal.pone.0240596. eCollection 2020.
To explore the underlying mechanism of dietary restriction (DR) induced lifespan extension in fruit flies at protein level, we performed proteome sequencing in Drosophila at day 7 (young) and day 42 (old) under DR and ad libitum (AL) conditions. A total of 18629 unique peptides were identified in Uniprot, corresponding to 3,662 proteins. Among them, 383 and 409 differentially expressed proteins (DEPs) were identified from comparison between DR vs AL at day 7 and 42, respectively. Bioinformatics analysis revealed that membrane-related processes, post-transcriptional processes, spliceosome and reproduction related processes, were highlighted significantly. In addition, expression of proteins involved in pathways such as spliceosomes, oxidative phosphorylation, lysosomes, ubiquitination, and riboflavin metabolism was relatively higher during DR. A relatively large number of DEPs were found to participate in longevity and age-related disease pathways. We identified 20 proteins that were consistently regulated during DR and some of which are known to be involved in ageing, such as mTORC1, antioxidant, DNA damage repair and autophagy. In the integration analysis, we found 15 genes that were stably regulated by DR at both transcriptional as well as translational levels. Our results provided a useful dataset for further investigations on the mechanism of DR and aging.
为了在蛋白质水平上探索饮食限制（DR）延长果蝇寿命的潜在机制，我们在 DR 和随意进食（AL）条件下，对第 7 天（年轻）和第 42 天（年老）的果蝇进行了蛋白质组测序。在 Uniprot 中鉴定出了 18629 个独特的肽，对应 3662 种蛋白质。其中，在第 7 天和第 42 天的 DR 与 AL 比较中，分别鉴定出 383 种和 409 种差异表达蛋白（DEPs）。生物信息学分析显示，膜相关过程、转录后过程、剪接体和生殖相关过程显著突出。此外，在 DR 期间，参与剪接体、氧化磷酸化、溶酶体、泛素化和核黄素代谢等途径的蛋白质表达相对较高。大量的 DEPs 被发现参与长寿和与年龄相关的疾病途径。我们鉴定出 20 种在 DR 期间持续调节的蛋白质，其中一些已知与衰老有关，如 mTORC1、抗氧化剂、DNA 损伤修复和自噬。在整合分析中，我们发现 15 个基因在转录和翻译水平都受到 DR 的稳定调节。我们的结果为进一步研究 DR 和衰老的机制提供了有用的数据集。
