黑腹果蝇的衰老研究。
Aging studies in Drosophila melanogaster.
作者信息
Sun Yaning, Yolitz Jason, Wang Cecilia, Spangler Edward, Zhan Ming, Zou Sige
机构信息
Translational Gerontology Branch, National Institute on Aging, Baltimore, MD, USA.
出版信息
Methods Mol Biol. 2013;1048:77-93. doi: 10.1007/978-1-62703-556-9_7.
Abstract
Drosophila is a genetically tractable system ideal for investigating the mechanisms of aging and developing interventions for promoting healthy aging. Here we describe methods commonly used in Drosophila aging research. These include basic approaches for preparation of diets and measurements of lifespan, food intake, and reproductive output. We also describe some commonly used assays to measure changes in physiological and behavioral functions of Drosophila in aging, such as stress resistance and locomotor activity.
摘要
果蝇是一种遗传上易于操作的系统，非常适合用于研究衰老机制以及开发促进健康衰老的干预措施。在此，我们描述果蝇衰老研究中常用的方法。这些方法包括制备食物的基本方法以及测量寿命、食物摄入量和生殖产出的方法。我们还描述了一些常用的测定方法，用于测量果蝇衰老过程中生理和行为功能的变化，如抗应激能力和运动活性。
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