Martínez-Pérez Raúl Balam, Rodríguez Jorge Alberto, Cira-Chávez Luis A, Dendooven Luc, Viniegra-González Gustavo, Estrada-Alvarado Isabel
Instituto Tecnológico de Sonora, 5 de febrero 818 sur, Col. Centro, 85000, Ciudad Obregón, SON, Mexico.
Centro de Investigación y Asistencia en Tecnología del Estado de Jalisco, Camino Arenero 1227, El Bajío del Arenal, 45019, Zapopan, JAL, Mexico.
Folia Microbiol (Praha). 2020 Oct;65(5):835-847. doi: 10.1007/s12223-020-00794-5. Epub 2020 May 15.
Halophilic bacterias from saline soil from former Lake Texcoco were isolated, identified based on 16 rRNA and tested to produce glucolytic, nucleolytic, proteolytic and lipolytic exoenzymes. The Bacillus, Virgibacillus, Kocuria, Salinicoccus, Gracilibacillus, Halobacillus, Tenuibacillus and Nesterekonia genera where identified. Lipase/eserases and proteases from Nesterenkonia sp. and Nesterenkonia aethiopica showed halotolerant characteristics and were selected to synthesize the oleochemical n-butyl oleate and antioxidant peptides from muscle protein of common carp (Cyprinus carpio), respectively. In organic media (2,2,4-Trimethylpentane), the lipase/esterases from Nesterenkonia sp. (0.6 U/mL) and N. aethiopica (1.2 U/mL) achieved a 62.7% and 53.2% of n-butyl oleate conversion, respectively. The protein hydrolysis from muscle of common carp (C. carpio) showed a degree of hydrolysis of 4.5 ± 0.2% and 2.8 ± 0.1% when proteases from Nesterenkonia sp. and N. aethiopica were used, respectively. Three peptidic fractions ranging molecular masses between 254 and 1002 Da [M + H] show antioxidant scavenging activity, and the principal fraction with a peptide of 547.3 Da [M + H] showed an inhibition of 37.7 ± 1.8% and 16.3 ± 0.6%, when 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) were used, respectively. These findings showed that the enzymatic battery of the halophilic bacteria from former lake Texcoco can be used in hydrolysis and synthesis of molecules with applications in different fields as food technology or bioenergy.
从特斯科科湖旧址的盐渍土中分离出嗜盐细菌，基于16 rRNA对其进行鉴定，并测试其产生糖解酶、核酸酶、蛋白酶和脂肪酶等胞外酶的能力。鉴定出了芽孢杆菌属、 Virgibacillus属、库克菌属、盐球菌属、纤细芽孢杆菌属、嗜盐芽孢杆菌属、薄壁芽孢杆菌属和涅斯捷连科氏菌属。涅斯捷连科氏菌属的某种菌株和埃塞俄比亚涅斯捷连科氏菌的脂肪酶/酯酶和蛋白酶表现出耐盐特性，分别被选用于合成油脂化学品油酸正丁酯和从鲤鱼（Cyprinus carpio）肌肉蛋白中制备抗氧化肽。在有机介质（2,2,4-三甲基戊烷）中，涅斯捷连科氏菌属的某种菌株（0.6 U/mL）和埃塞俄比亚涅斯捷连科氏菌（1.2 U/mL）的脂肪酶/酯酶分别实现了62.7%和53.2%的油酸正丁酯转化率。当分别使用涅斯捷连科氏菌属的某种菌株和埃塞俄比亚涅斯捷连科氏菌的蛋白酶时，鲤鱼（C. carpio）肌肉的蛋白质水解度分别为4.5±0.2%和2.8±0.1%。三个分子量在254至1002 Da [M + H]之间的肽段显示出抗氧化清除活性，当分别使用2,2-二苯基-1-苦基肼和2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)时，主要的含547.3 Da [M + H]肽段的部分分别表现出37.7±1.8%和16.3±0.6%的抑制率。这些发现表明，来自特斯科科湖旧址的嗜盐细菌的酶系可用于水解和合成在食品技术或生物能源等不同领域有应用的分子。
