Maragou Niki C, Makri Athina, Lampi Eugenia N, Thomaidis Nikolaos S, Koupparis Michael A
Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 157 71 Athens, Greece.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Mar;25(3):373-83. doi: 10.1080/02652030701509998.
Migration of the potential endocrine disrupter, bisphenol A (BPA), from 31 polycarbonate (PC) baby bottles into aqueous food simulants was studied under real repetitive use, using a sensitive and fully validated liquid chromatographic method with fluorescence detection. Confirmation of the presence of BPA was performed by liquid chromatography-mass spectrometry (LC-MS). The effects of cleaning in a dishwasher or with a brush, sterilization with boiling water and the temperature of migration were examined. It was shown that temperature was the crucial factor for the migration of BPA from the plastic bottles to water. All samples released BPA in the concentration range 2.4-14.3 microg kg(-1) when filled with boiled water and left at ambient temperature for 45 min. The decrease of BPA release in the sterilization water and in the food simulant over 12 cycles of use indicated that the hypothesis of polymer degradation in water is dubious. Estimated infantile dietary exposure, regarding the use of PC baby bottles, ranged between 0.2 and 2.2 microg kg(-1) bw day(-1), which is below the Tolerable Daily Intake of 50 microg kg(-1) bw recently established by EFSA.
采用灵敏且经过充分验证的带荧光检测的液相色谱法，研究了在实际重复使用情况下，潜在内分泌干扰物双酚A（BPA）从31个聚碳酸酯（PC）婴儿奶瓶向水性食品模拟物中的迁移情况。通过液相色谱-质谱联用（LC-MS）对双酚A的存在进行确认。考察了洗碗机清洗或刷子刷洗、沸水消毒以及迁移温度的影响。结果表明，温度是双酚A从塑料奶瓶向水中迁移的关键因素。当装满沸水并在室温下放置45分钟时，所有样品释放的双酚A浓度范围为2.4 - 14.3微克/千克（-1）。经过12个使用周期后，消毒水中和食品模拟物中双酚A释放量的减少表明，聚合物在水中降解的假设值得怀疑。关于使用PC婴儿奶瓶，估计婴儿膳食暴露量在0.2至2.2微克/千克体重·天（-1）之间，低于欧洲食品安全局（EFSA）最近确定的每日可耐受摄入量50微克/千克体重。
