State Key Laboratory for Hubei New Textile Materials and Advanced Processing Technologies, School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430200, China.
State Key Laboratory for Hubei New Textile Materials and Advanced Processing Technologies, School of Textile Science and Engineering, Wuhan Textile University, Wuhan, 430200, China.
Carbohydr Polym. 2020 Jul 1;239:116232. doi: 10.1016/j.carbpol.2020.116232. Epub 2020 Apr 6.
Combining the properties of natural protein and polysaccharide is a promising strategy to generate bioactive biomaterials with controlled structure. Here, a new method of preparing water-insoluble silk fibroin/hyaluronic acid (SF/HA) scaffolds with tunable performances using an all-aqueous process is reported. Freezing-induced assembly was used to form silk I crystallization in the SF/HA blends. Silk I crystallization enhanced the stability of SF/HA scaffolds in water by forming silk I crystal networks to entrap blended HA without chemical cross-linking. Increasing HA content significantly enhanced the flexibility and water binding capacity of porous scaffolds, but high amount of HA reduced the water-stability of porous scaffolds due to insufficient silk I crystal cross-links. The enzymatic degradation behavior of the SF/HA scaffolds was investigated, revealing that the regulation ability of HA in the SF scaffolds. This novel nonchemically cross-linked protein/polysaccharide scaffold may be useful for soft tissue engineering due to excellent biocompatibility and tunable performances.
将天然蛋白质和多糖的特性结合起来，是生成具有可控结构的生物活性生物材料的一种很有前途的策略。在这里，报道了一种新的方法，即使用全水相工艺制备具有可调性能的不溶于水的丝素蛋白/透明质酸（SF/HA）支架。冷冻诱导组装用于在 SF/HA 共混物中形成丝素 I 结晶。丝素 I 结晶通过形成丝素 I 晶体网络来捕获混合的 HA，而无需化学交联，从而增强了 SF/HA 支架在水中的稳定性。HA 含量的增加显著提高了多孔支架的柔韧性和水结合能力，但由于丝素 I 晶体交联不足，高含量的 HA 降低了多孔支架的水稳定性。还研究了 SF/HA 支架的酶降解行为，揭示了 HA 在 SF 支架中的调节能力。由于具有优异的生物相容性和可调性能，这种新型的非化学交联的蛋白质/多糖支架可能可用于软组织工程。
