Department of Physical Education, Ludong University, City Yantai 264025, Shan Dong Province, China
Department of Physical Education, Ludong University, City Yantai 264025, Shan Dong Province, China.
Biol Open. 2020 May 29;9(5):bio045260. doi: 10.1242/bio.045260.
A high-salt diet (HSD) is a major cause of many chronic and age-related defects such as myocardial hypertrophy, locomotor impairment and mortality. Exercise training can efficiently prevent and treat many chronic and age-related diseases. However, it remains unclear whether endurance exercise can resist HSD-induced impairment of climbing capacity and longevity in aging individuals. In our study, flies were given exercise training and fed a HSD from 1-week old to 5-weeks old. Overexpression or knockdown of and were built by UAS/Gal4 system. The results showed that a HSD, gene overexpression and knockdown significantly reduced climbing endurance, climbing index, survival, expression and SOD activity level, and increased malondialdehyde level in aging flies. Inversely, in a HSD aging flies, endurance exercise and overexpression significantly increased their climbing ability, lifespan and antioxidant capacity, but they did not significantly change the gene expression. Overall, current results indicated that a HSD accelerated the age-related decline of climbing capacity and mortality via upregulating expression and inhibiting the /SOD pathway. Increased /SOD pathway activity played a key role in mediating endurance exercise resistance to the low salt tolerance-induced impairment of climbing capacity and longevity in aging This article has an associated First Person interview with the first author of the paper.
高盐饮食（HSD）是许多慢性和与年龄相关的缺陷的主要原因，如心肌肥大、运动障碍和死亡率。运动训练可以有效地预防和治疗许多慢性和与年龄相关的疾病。然而，目前尚不清楚耐力运动是否能抵抗 HSD 诱导的衰老个体攀爬能力和寿命的降低。在我们的研究中，从 1 周龄到 5 周龄，用 HSD 喂养苍蝇，并进行运动训练。通过 UAS/Gal4 系统构建了 和 的过表达或敲低。结果表明，HSD、 基因过表达和 敲低显著降低了衰老苍蝇的攀爬耐力、攀爬指数、存活率、 表达和 SOD 活性水平，并增加了丙二醛水平。相反，在 HSD 衰老苍蝇中，耐力运动和 过表达显著提高了它们的攀爬能力、寿命和抗氧化能力，但它们对 基因表达没有显著影响。总的来说，目前的结果表明，HSD 通过上调 表达和抑制 /SOD 途径加速了与年龄相关的攀爬能力下降和死亡率的增加。/SOD 途径活性的增加在介导耐力运动抵抗低盐耐受性诱导的衰老个体攀爬能力和寿命降低方面起着关键作用。本文有与论文第一作者的相关第一人称采访。
