Yu Shi-Jiang, Cong Lin, Liu Hao-Qiang, Ran Chun
Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, National Engineering Research Center for Citrus, Chongqing400712, China.
Bull Entomol Res. 2020 Dec;110(6):743-755. doi: 10.1017/S0007485320000267. Epub 2020 May 18.
Panonychus citri (McGregor) is the most common pest in citrus-producing regions. Special low-toxicity acaricides, such as spirocyclic tetronic acids and mite growth inhibitors, have been used for a long time in China. However, pesticide resistance in mites is a growing problem due to the lack of new acaricide development. Wide-spectrum insecticides, such as amitraz have gained acceptance among fruit growers. An amitraz-resistant strain of P. citri was obtained by indoor screening to examine field resistance monitoring of mites to acaricides and to explore the resistant mechanism of mites against amitraz. The amitraz-resistant strain of P. citri had an LC50 value of 2361.45 mg l-1. The resistance ratio was 81.35 times higher in the resistant strain of P. citri compared with the sensitive strain. Crossing experiments between the sensitive and resistant strains of P. citri were conducted, resulting in a D value of 0.11 for F1 SS♀×RS♂ and 0.06 for F1 RS♀×SS♂. Reciprocal cross experiments showed that the dose-mortality curves for the F1 generations coincided, indicating that the resistance trait was not affected by cytoplasmic inheritance. The dose-expected response relationship was evaluated in the backcross generation and a significant difference was observed compared with the actual value. The above results indicate that the inheritance of resistance trait was incompletely dominant, governed by polygenes on the chromosome. Synergism studies demonstrated that cytochrome P450s and esterase may play important roles in the detoxification of amitraz. Based on differential gene analysis, 23 metabolism-related genes of P. citri were identified, consistent with the results of synergism studies. Real-time PCR verification implied that P450s, ABC transporters, and acetylcholinesterase might influence the detoxification of amitraz by P. citri. These results provide the genetic and molecular foundation for the management of pest mite resistance.
柑橘全爪螨（麦格雷戈）是柑橘产区最常见的害虫。在中国，诸如螺环四酮酸类和杀螨生长抑制剂等特殊低毒杀螨剂已被长期使用。然而，由于缺乏新的杀螨剂研发，螨类对农药的抗性问题日益严重。诸如双甲脒等广谱杀虫剂已在果农中得到认可。通过室内筛选获得了柑橘全爪螨的双甲脒抗性品系，以检测螨类对杀螨剂的田间抗性监测，并探索螨类对双甲脒的抗性机制。柑橘全爪螨的双甲脒抗性品系的LC50值为2361.45 mg l-1。抗性品系的抗性倍数比敏感品系高81.35倍。对柑橘全爪螨的敏感品系和抗性品系进行了杂交试验，F1 SS♀×RS♂的D值为0.11，F1 RS♀×SS♂的D值为0.06。正反交试验表明，F1代的剂量-死亡率曲线重合，表明抗性性状不受细胞质遗传的影响。在回交世代中评估了剂量-预期反应关系，与实际值相比观察到显著差异。上述结果表明，抗性性状的遗传是不完全显性的，由染色体上的多基因控制。增效作用研究表明，细胞色素P450和酯酶可能在双甲脒的解毒中起重要作用。基于差异基因分析，鉴定出23个柑橘全爪螨的代谢相关基因，与增效作用研究结果一致。实时荧光定量PCR验证表明，P45色体上的多基因控制。增效作用研究表明，细胞色素P450和酯酶可能在双甲脒的解毒中起重要作用。基于差异基因分析，鉴定出23个柑橘全爪螨的代谢相关基因，与增效作用研究结果一致。实时荧光定量PCR验证表明，P450、ABC转运蛋白和乙酰胆碱酯酶可能影响柑橘全爪螨对双甲脒的解毒。这些结果为害螨抗性治理提供了遗传和分子基础。 0、ABC转运蛋白和乙酰胆碱酯酶可能影响柑橘全爪螨对双甲脒的解毒。这些结果为害螨抗性治理提供了遗传和分子基础。
