Seaman Josiah, Buggs Richard J A
Royal Botanic Gardens Kew, Jodrell Laboratory, Richmond, United Kingdom.
School of Biological and Chemical Sciences, Queen Mary University of London, London, United Kingdom.
Front Genet. 2020 Apr 30;11:292. doi: 10.3389/fgene.2020.00292. eCollection 2020.
Researchers seldom look at naked genome assemblies: instead the attributes of DNA sequences are mediated through statistics, annotations and high level summaries. Here we present software that visualizes the bare sequences of whole genome assemblies in a zoomable interface. This can assist in detection of chromosome architecture and contamination by the naked eye through changes in color patterns, in the absence of any other annotation. When available, annotations can be visualized alongside or on top of the naked sequence. Genome alignments can also be visualized, laying two genomes side by side in an alignment and highlighting their differences at nucleotide resolution. FluentDNA gives researchers direct visualization of whole genome assemblies, annotations and alignments, for quality control, hypothesis generation, and communicating results.
相反，DNA序列的特征是通过统计、注释和高级摘要来体现的。在此，我们展示一款软件，它能在可缩放界面中可视化全基因组组装的原始序列。在没有任何其他注释的情况下，这有助于通过颜色模式的变化，肉眼检测染色体结构和污染情况。如有可用注释，也可与原始序列一起或在其上方进行可视化展示。基因组比对同样可以可视化，将两个基因组并排排列进行比对，并在核苷酸分辨率下突出显示它们的差异。FluentDNA使研究人员能够直接可视化全基因组组装、注释和比对结果，用于质量控制、假设生成以及结果交流。
