Chen Jiawei, Liang Linying, Li Yanan, Zhang Haibin
Institute of Deep-sea Science and Engineering, Chinese Academy of Sciences, Sanya, China.
College of Earth Sciences, University of Chinese Academy of Sciences, Beijing, China.
Front Genet. 2020 Apr 30;11:355. doi: 10.3389/fgene.2020.00355. eCollection 2020.
Hydrostatic pressure is a key environmental factor constraining the benthic migration of shallow-water invertebrates. Although many studies have examined the physiological effects of high hydrostatic pressure on shallow-water invertebrates, the molecular response to high pressure is not fully understood. This question has received increasing attention because ocean warming is forcing the bathymetric migrations of shallow-water invertebrates. Here, we applied time-series transcriptomic analysis to high-pressure incubated and atmospheric pressure-recovered shallow-water sea cucumber () to address this question. A total of 44 samples from 15 experimental groups were sequenced. Our results showed that most genes responded to pressure stress at the beginning when pressure was changed, but significant differences of gene expression appeared after 4 to 6 h. Transcription was the most sensitive biological process responding to high-pressure exposure, which was enriched among up-regulated genes after 2 h, followed by ubiquitination (4 h), endocytosis (6 h), stress response (6 h), methylation regulation (24 h), and transmembrane transportation (24 h). After high-pressure incubation, all these biological processes remained up-regulated within 4-6 h at atmospheric pressure. Overall, our results revealed the dynamic transcriptional response of to high-pressure exposure. Additionally, few quantitative or functional responses related to on transcriptional level were introduced by hydrostatic pressure changes after 1 h, and main biological responses were introduced after 4 h, suggesting that, when hydrostatic pressure is the mainly changed environmental factor, it will be better to fix sea cucumber samples for transcriptomic analysis within 1 h, but 4 h will be also acceptable.
静水压力是限制浅水无脊椎动物底栖迁移的关键环境因素。尽管许多研究已经考察了高静水压力对浅水无脊椎动物的生理影响，但对高压的分子反应尚未完全了解。由于海洋变暖正迫使浅水无脊椎动物进行深度迁移，这个问题受到了越来越多的关注。在这里，我们对高压处理和常压恢复后的浅水海参进行了时间序列转录组分析，以解决这个问题。对来自15个实验组的总共44个样本进行了测序。我们的结果表明，大多数基因在压力变化开始时对压力应激有反应，但基因表达的显著差异在4至6小时后出现。转录是对高压暴露最敏感的生物学过程，在2小时后上调的基因中富集，其次是泛素化（4小时）、内吞作用（6小时）、应激反应（6小时）、甲基化调控（24小时）和跨膜运输（24小时）。高压处理后，在常压下，所有这些生物学过程在4 - 6小时内保持上调。总体而言，我们的结果揭示了海参对高压暴露的动态转录反应。此外，静水压力变化在1小时后在转录水平上很少引入与海参相关的定量或功能反应，主要生物学反应在4小时后出现，这表明，当静水压力是主要变化的环境因素时，在1小时内固定海参样本进行转录组分析会更好，但4小时也是可以接受的。
