Do Minh Duc, Le Linh Gia Hoang, Nguyen Vinh The, Dang Tran Ngoc, Nguyen Nghia Hoai, Vu Hoang Anh, Mai Thao Phuong
Center for Molecular Biomedicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
Faculty of Public Health, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam.
Front Genet. 2020 Apr 30;11:383. doi: 10.3389/fgene.2020.00383. eCollection 2020.
Human leukocyte antigen (HLA) genotyping displays the particular characteristics of HLA alleles and haplotype frequencies in each population. Although it is considered the current gold standard for HLA typing, high-resolution sequence-based HLA typing is currently unavailable in Kinh Vietnamese populations. In this study, high-resolution sequence-based HLA typing (3-field) was performed using an amplicon-based next-generation sequencing platform to identify the HLA-A, -B, -C, -DRB1, and -DQB1 alleles of 101 unrelated healthy Kinh Vietnamese individuals from southern Vietnam. A total of 28 HLA-A, 41 HLA-B, 21 HLA-C, 26 HLA-DRB1, and 25 HLA-DQB1 alleles were identified. The most frequently occurring HLA alleles were A11:01:01, B15:02:01, C07:02:01, DRB112:02:01, and DQB103:01:01. Haplotype calculation showed that A29:01:01∼B07:05:01, DRB112:02:01∼DQB13:01:01, A29:01:01∼C15:05:02∼B07:05:01, A33:03:01∼B58:01:01∼DRB103:01:01, and A29:01:01∼C15:05:02∼B07:05:01∼DRB110:01:01∼DQB105:01:01 were the most common haplotypes in the southern Kinh Vietnamese population. Allele distribution and haplotype analyses demonstrated that the Vietnamese population shares HLA features with South-East Asians but retains unique characteristics. Data from this study will be potentially applicable in medicine and anthropology.
人类白细胞抗原（HLA）基因分型显示了各人群中HLA等位基因和单倍型频率的特定特征。尽管它被认为是目前HLA分型的金标准，但在京族越南人群中目前尚无基于高分辨率序列的HLA分型。在本研究中，使用基于扩增子的下一代测序平台进行了基于高分辨率序列的HLA分型（3字段），以鉴定来自越南南部的101名无亲缘关系的健康京族越南个体的HLA - A、-B、-C、-DRB1和-DQB1等位基因。共鉴定出28个HLA - A、41个HLA - B、21个HLA - C、26个HLA - DRB1和25个HLA - DQB1等位基因。最常见的HLA等位基因是A11:01:01、B15:02:01、C07:02:01、DRB112:02:01和DQB103:01:01。单倍型计算表明，A29:01:01∼B07:05:01、DRB112:02:01∼DQB13:01:01、A29:01:01∼C15:05:02∼B07:05:01、A33:03:01∼B58:01:01∼DRB103:01:01以及A29:01:01∼C15:05:02∼B07:05:01∼DRB110:01:01∼DQB105:01:01是越南南部京族人群中最常见的单倍型。等位基因分布和单倍型分析表明，越南人群与东南亚人群共享HLA特征，但保留了独特的特性。本研究的数据可能在医学和人类学中具有应用价值。
