Vu-Trieu A, Djoulah S, Tran-Thi C, Ngyuyen-Thanh T, Le Monnier De Gouville I, Hors J, Sanchez-Mazas A
Department of Immunology and Physiopathology, Medical College of Hanoi, Vietnam.
Eur J Immunogenet. 1997 Oct;24(5):345-56. doi: 10.1046/j.1365-2370.1997.d01-107.x.
We report here the DNA polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) typing of the HLA-DR B1, B3, B4, B5 and DQB1 loci for a sample of 103 Vietnamese Kinh from Hanoi, and compare their allele and haplotype frequencies to other East Asiatic and Oceanian populations studied during the 11th and 12th International HLA Workshops. The Kinh exhibit some very high-frequency alleles both at DRB1 (1202, which has been confirmed by DNA sequencing, and 0901) and DQB1 (0301, 03032, 0501) loci, which make them one of the most homogeneous population tested so far for HLA class II in East Asia. Three haplotypes account for almost 50% of the total haplotype frequencies in the Vietnamese. The most frequent haplotype is HLA-DRB11202-DRB30301-DQB10301 (28%), which is also predominant in Southern Chinese, Micronesians and Javanese. On the other hand, DRB11201 (frequent in the Pacific) is virtually absent in the Vietnamese. The second most frequent haplotype is DRB10901-DRB401011-DQB103032 (14%), which is also commonly observed in Chinese populations from different origins, but with a different accessory chain (DRB40301) in most ethnic groups. Genetic distances computed for a set of Asiatic and Oceanian populations tested for DRB1 and DQB1 and their significance indicate that the Vietnamese are close to the Thai, and to the Chinese from different locations. These results, which are in agreement with archaeological and linguistic evidence, contribute to a better understanding of the origin of the Vietnamese population, which has until now not been clear.
我们在此报告对来自河内的103名越南京族样本进行的HLA - DR B1、B3、B4、B5和DQB1基因座的DNA聚合酶链反应序列特异性寡核苷酸（PCR - SSO）分型，并将其等位基因和单倍型频率与在第11届和第12届国际HLA研讨会上研究的其他东亚和大洋洲人群进行比较。京族在DRB1（经DNA测序确认的1202以及0901）和DQB1（0301、03032、0501）基因座上表现出一些高频等位基因，这使他们成为迄今为止东亚地区HLA - II类检测中最同质化的人群之一。三种单倍型几乎占越南人总单倍型频率的50%。最常见的单倍型是HLA - DRB11202 - DRB30301 - DQB10301（28%），在中国南方人、密克罗尼西亚人和爪哇人中也占主导地位。另一方面，DRB11201（在太平洋地区常见）在越南人中几乎不存在。第二常见的单倍型是DRB10901 - DRB401011 - DQB103032（14%），在不同来源的中国人群中也普遍观察到，但在大多数族群中具有不同的辅助链（DRB40301）。针对一组检测了DRB1和DQB1的亚洲和大洋洲人群计算的遗传距离及其显著性表明，越南人与泰国人以及来自不同地区的中国人关系密切。这些结果与考古学和语言学证据一致，有助于更好地理解越南人群的起源，而越南人群的起源至今尚不清楚。
