Department of Biomedical Science and Engineering Physics and Photon Science, Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Republic of Korea.
Department of Bioengineering, College of Engineering, and BK21 PLUS Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University, Seoul, 04763, Republic of Korea.
Sci Rep. 2020 May 19;10(1):8254. doi: 10.1038/s41598-020-65103-z.
Glucose level is a primary indicator in the diagnosis and treatment of diabetes mellitus. According to the correlation between glucose concentration in blood and tears, measuring tear glucose can be an alternative to traditional strips test for blood glucose. Thus, measuring tear glucose levels could provide noninvasive monitoring of blood glucose. As a biocompatible biosensor, a nanoparticle embedded contact lens (NECL) is developed which is composed of glucose oxidase and cerium oxide (III). Using spectroscopy, we found the detectable changes in reflection spectrum of contact lenses with respect to the glucose concentration, and developed correlation curve of the reflection spectrum with known glucose level. Furthermore, we assessed tear glucose level and compared blood glucose level with the diabetic mouse model to evaluate this approach. Our algorithm for regular monitoring of glucose using contact lens biosensor may lead to noninvasive monitoring of tear glucose level. NECL may provide simple and noninvasive glucose monitoring based on the spectral changes in contact lens biosensor.
血糖水平是诊断和治疗糖尿病的主要指标。根据血液和泪液中葡萄糖浓度的相关性，测量泪液中的葡萄糖可以替代传统的血糖试纸检测。因此，测量泪液中的葡萄糖水平可以提供对血糖的无创监测。作为一种生物相容性的生物传感器，我们开发了一种纳米粒子嵌入隐形眼镜（NECL），它由葡萄糖氧化酶和氧化铈（III）组成。通过光谱学，我们发现了接触镜反射光谱随葡萄糖浓度的可检测变化，并开发了反射光谱与已知葡萄糖水平的相关曲线。此外，我们评估了糖尿病小鼠模型中的泪液葡萄糖水平，并与血糖水平进行了比较，以评估这种方法。我们使用隐形眼镜生物传感器的算法进行常规的血糖监测可能会导致对泪液葡萄糖水平的无创监测。NECL 可能基于接触镜生物传感器的光谱变化提供简单和无创的葡萄糖监测。
