Department of Ophthalmology, Keio University School of Medicine, Tokyo 160-8582, Japan.
i.com medical GmbH, 81241 Munich, Germany.
Int J Mol Sci. 2020 May 15;21(10):3516. doi: 10.3390/ijms21103516.
Hyaluronic acid (HA) ophthalmic solution is widely used in dry eye treatment worldwide. However, there are no reports comparing the dry eye treatment effects of high molecular weight HA with low molecular weight HA. Sixty eight-week-old C57BL/6 mice were assigned to the following 6 groups and exposed to environmental dry eye stress (EDES) that mimics office work environment: 1) 0.1% low molecular weight HA (LMWHA) eye drops, 2) 0.3% LMWHA eye drops, 3) 3% diquafosol sodium (DQ) eye drops, 4) 0.15% high molecular weight HA (HMWHA) eye drops, 5) no treatment with exposure to EDES (EDES+/Treatment-), and 6) no treatment without exposure to EDES (EDES-/Treatment-). After EDES, the HMWHA group had significantly longer break-up time (BUT) than the 0.1%, 0.3% LMWHA groups and the DQ group. After EDES, the HMWHA group had significantly lower lissamine green staining scores than the LMWHA and DQ groups. Subepithelial presumed dendritic cell density in the HMWHA group was significantly lower than the EDES+/Treatment- group. After EDES exposure, Conjunctival Muc5AC mRNA expression in the HMWHA group was significantly higher than the 0.1 and 0.3% LMWHA groups. Ophthalmic HMWHA solution may have a better dry eye treatment effect than LMWHA or DQ solution, owing to its anti-inflammatory effect.
玻璃酸钠（HA）滴眼液在全球范围内广泛用于干眼症的治疗。然而，目前尚无研究比较高分子量 HA 和低分子量 HA 治疗干眼症的效果。将 68 周龄 C57BL/6 小鼠分为以下 6 组，并使其暴露于模拟办公环境的环境干燥眼应激（EDES）中：1）0.1%低分子量 HA（LMWHA）滴眼液，2）0.3%LMWHA 滴眼液，3）3%双氯非腙钠（DQ）滴眼液，4）0.15%高分子量 HA（HMWHA）滴眼液，5）EDES 暴露但不治疗（EDES+/Treatment-），6）无 EDES 暴露且不治疗（EDES-/Treatment-）。EDES 后，HMWHA 组的泪膜破裂时间（BUT）明显长于 0.1%、0.3%LMWHA 组和 DQ 组。EDES 后，HMWHA 组的丽丝胺绿染色评分明显低于 LMWHA 和 DQ 组。HMWHA 组的上皮下假定树突状细胞密度明显低于 EDES+/Treatment-组。EDES 暴露后，HMWHA 组结膜 Muc5ACmRNA 表达明显高于 0.1%和 0.3%LMWHA 组。眼科用 HMWHA 溶液可能比 LMWHA 或 DQ 溶液具有更好的干眼症治疗效果，这归因于其抗炎作用。
