Institute of Soil and Water Conservation, Chinese Academy of Sciences & Ministry of Water Resource, Yangling 712100, China.
University of the Chinese Academy of Sciences, Beijing 100049, China.
Genes (Basel). 2020 May 16;11(5):557. doi: 10.3390/genes11050557.
is a well-known Chinese herb for the clinical treatment of cardiovascular and cerebrovascular diseases. Tanshinones and phenolic acids are the major secondary metabolites and significant pharmacological constituents of this plant. Kelch repeat F-box (KFB) proteins play important roles in plant secondary metabolism, but their regulation mechanism in has not been characterized. In this study, we systematically characterized the KFB gene family. In total, 31 genes were isolated from . Phylogenetic analysis of those indicated that 31 can be divided into four groups. Thereinto, five () shared high homology with other plant KFBs which have been described to be regulators of secondary metabolism. The expression profile of under methyl jasmonate (MeJA) treatment deciphered that six () were significantly downregulated, and two () were significantly upregulated. Tissue-specific expression analysis found that four () were expressed preferentially in aerial tissues, while two () were predominantly expressed in roots. Through a systematic analysis, we speculated that are potentially involved in phenolic acids biosynthesis.
丹参是一种常用于治疗心脑血管疾病的中药。丹参酮和酚酸类化合物是该植物的主要次生代谢产物和重要的药理成分。Kelch 重复 F-box（KFB）蛋白在植物次生代谢中发挥着重要作用，但它们在 中的调控机制尚未得到阐明。本研究系统地对 中的 KFB 基因家族进行了鉴定。从 中总共分离出 31 个基因。对这些基因的系统发育分析表明，31 个基因可分为四个亚组。其中，五个 （）与其他已被描述为次生代谢调节剂的植物 KFB 具有高度同源性。茉莉酸甲酯（MeJA）处理下的 表达谱分析表明，有六个 （）显著下调，两个 （）显著上调。组织特异性表达分析发现，四个 （）在地上组织中优先表达，而两个 （）主要在根部表达。通过系统分析，我们推测 可能参与了酚酸类物质的生物合成。
