Chen Chengan, Wang Can, Li Junbo, Gao Xiankui, Huang Qikai, Gong Yifu, Hao Xiaolong, Maoz Itay, Kai Guoyin, Zhou Wei
Laboratory for Core Technology of TCM Quality Improvement and Transformation, School of Pharmaceutical Sciences, The Third Affiliated Hospital, School of Pharmacy and Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.
Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Rishon LeZion, Israel.
Front Plant Sci. 2022 Feb 9;13:829447. doi: 10.3389/fpls.2022.829447. eCollection 2022.
Plant U-box (PUB) proteins are ubiquitin ligases (E3) involved in multiple biological processes and in response to plant stress. However, the various aspects of the genome and the differences in functions between the U-box E3 (UBE3) ubiquitin ligases remain quite obscure in . The 60 genes in the genome were recognized in the present study. The phylogenetic analysis, gene structure, motifs, promoters, and physical and chemical properties of the genes were also examined. Based on the phylogenetic relationship, the 60 genes were categorized under six different groups. The U-box domain was highly conserved across the family of genes. Analysis of the -acting element revealed that the genes might play an important role in a variety of biological processes, including a reaction to the abscisic acid (ABA) treatment. To investigate this hypothesis, an ABA treatment was developed for the hairy roots of . Thirteen out of the genes significantly increased after the ABA treatment. The co-expression network revealed that nine genes might be associated with phenolic acids or tanshinone biosynthesis. The findings of the present study brought fresh and new understanding to the participation of the gene family in plants, specifically in their biological responses mediated by the ABA. In , this gene family may be crucial during the ABA treatment. Significantly, the results of this study contribute novel information to the understanding of the ubiquitin ligase gene and its role in plant growth.
植物U-box（PUB）蛋白是参与多种生物学过程及植物应激反应的泛素连接酶（E3）。然而，U-box E3（UBE3）泛素连接酶在基因组的各个方面以及功能差异在[具体物种]中仍相当模糊。本研究识别出了[具体物种]基因组中的60个该类基因。还对这些基因的系统发育分析、基因结构、基序、启动子以及理化性质进行了研究。基于系统发育关系，这60个基因被分为六个不同的组。U-box结构域在该类基因家族中高度保守。对顺式作用元件的分析表明，这些基因可能在多种生物学过程中发挥重要作用，包括对脱落酸（ABA）处理的反应。为了验证这一假设，对[具体物种]的毛状根进行了ABA处理。在ABA处理后，60个基因中的13个显著增加。共表达网络显示，9个基因可能与酚酸或丹参酮生物合成相关。本研究结果为该基因家族在植物中的参与情况，特别是在由ABA介导的生物学反应中的参与情况带来了新的认识。在[具体物种]中，这个基因家族在ABA处理过程中可能至关重要。重要的是，本研究结果为理解泛素连接酶基因及其在植物生长中的作用提供了新的信息。
