Sun Haibing, Chu Shanshan, Jiang Lu, Tong Zhenzhen, Cheng Ming'en, Peng Huasheng, Huang Luqi
School of Pharmacy, Anhui University of Chinese Medicine, Hefei, China.
State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Front Plant Sci. 2023 Aug 4;14:1179915. doi: 10.3389/fpls.2023.1179915. eCollection 2023.
The genus L. (Apiaceae) is a large group comprising more than 120 species distributed worldwide. Many plants of the genus have been studied and used in traditional Chinese medicine. In 2020, a new species, Lu Q. Huang, H. S. Peng & S. S. Chu, was found in the Huangshan Mountains of Anhui Province, China. However, little is known about its medicinal properties. Thus, the objective of this study is to explore the potential medicinal value of . and its relationship with other species. Through textual research on illustrations of in Bencao literature, it can be inferred that at least five species of genus have been used in Chinese medicine. Therefore, we chose these five species of and . together for subsequent research. We conducted morphological, chloroplast genome, and chemical analyses of six species, including the newly discovered . The chloroplast genomes of showed a typical tetrad structure, and the gene structure and content were similar and conservative. There were significant differences in genome size and the expansion of the inverted repeat boundary. Through nucleotide polymorphism analysis, we screened 14 hotspot mutation regions that have the potential to be used as specific molecular markers for the taxonomy of . Our results showed an inversion of the gene in the chloroplast genome, which can be developed as a specific molecular marker for species identification. Phylogenetic analysis showed that the phylogenetic trees had high support and resolution, which strongly supports the view that is not a monophyletic group. had the closest genetic relationship to K. T. Fu, followed by Fedde ex Wolff. Furthermore, the main coumarins of were most similar to those of Thunb. and . In summary, our research lays a foundation for the systematic classification of and sheds light on the medicinal value of .
当归属（伞形科）是一个大属，包含120多种分布于世界各地的物种。该属的许多植物已被研究并应用于传统中药。2020年，在中国安徽省黄山发现了一个新物种，即黄璐琦、彭华胜和储珊珊命名的[具体新物种名称未给出]。然而，对其药用特性知之甚少。因此，本研究的目的是探索[具体物种名称未给出]的潜在药用价值及其与当归属其他物种的关系。通过对本草文献中当归属插图的考证，可以推断至少有五种当归属植物已被用于中药。因此，我们选择了这五种当归属植物以及[具体物种名称未给出]一起进行后续研究。我们对六个当归属物种进行了形态学、叶绿体基因组和化学分析，其中包括新发现的[具体物种名称未给出]。当归属植物的叶绿体基因组呈现典型的四分体结构，基因结构和内容相似且保守。基因组大小和反向重复边界的扩展存在显著差异。通过核苷酸多态性分析，我们筛选出14个热点突变区域，这些区域有可能用作当归属分类学的特定分子标记。我们的结果表明，[具体物种名称未给出]叶绿体基因组中的[具体基因名称未给出]基因发生了倒位，可将其开发为物种鉴定的特定分子标记。系统发育分析表明，系统发育树具有较高的支持率和分辨率，有力地支持了当归属不是单系类群的观点。[具体物种名称未给出]与傅氏当归属（K. T. Fu）的亲缘关系最近，其次是少花当归（Fedde ex Wolff）。此外，[具体物种名称未给出]的主要香豆素与白花前胡（Peucedanum praeruptorum）和紫花前胡（Peucedanum decursivum）的最为相似。总之，我们的研究为当归属的系统分类奠定了基础，并揭示了[具体物种名称未给出]的药用价值。
