Breen E J, Williams K L
School of Biological Sciences, Macquarie University, Sydney, Australia.
Dev Genet. 1988;9(4-5):539-48. doi: 10.1002/dvg.1020090430.
The last 5 years have resulted in many advances in knowledge of the cytoskeleton and motility of individual cells. Here the problem of multicellular movement is addressed. The Dictyostelium discoideum slug is examined, and models for how approximately 100,000 cells become coordinated to move are briefly reviewed. Experiments that contributed to model building as well as those used to test models are considered. Four levels of experimentation are considered: (1) the extracellular matrix (ECM) is examined as a component of the system; (2) information obtained by examining the organisation of slug cells through sectioning is presented; (3) time, the 4th dimension, is considered, and approaches to studying the dynamics of cell interactions from the point of view of movement are outlined, and (4) cell adhesion molecules are addressed.
过去五年里，我们对单个细胞的细胞骨架和运动性有了许多新的认识。本文探讨多细胞运动的问题。我们研究了盘基网柄菌蛞蝓体，并简要回顾了约10万个细胞如何协同移动的模型。文中考虑了有助于模型构建的实验以及用于测试模型的实验。我们考虑了四个实验层面：（1）将细胞外基质（ECM）作为系统的一个组成部分进行研究；（2）展示通过切片研究蛞蝓体细胞组织所获得的信息；（3）考虑时间这一第四维度，并概述从运动角度研究细胞相互作用动态的方法；（4）探讨细胞黏附分子。
