Tian Zhenqi, Ma Chao, Zhang Yan, Chen Hongsong, Gao Xuyuan, Guo Jianying, Zhou Zhongshi
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya, China.
Front Plant Sci. 2023 Jul 17;14:1114026. doi: 10.3389/fpls.2023.1114026. eCollection 2023.
Low temperatures greatly influence newly introduced species, and increased cold tolerance can facilitate their establishment in new environments. The invasive alien species is distributed at high latitudes and altitudes, where it suffers more from cold stress than it would at low latitudes or altitudes. Whether cold stress influences the accumulation of cryoprotectants and cold tolerance in , and further influences the cold tolerance of its biological control agent, , through feeding remain unknown. We investigated the levels of cryoprotectants and metabolic changes in We found that the level of total sugar, trehalose, proline, and other cold responsible metabolites increased in after rapid cold-hardening (RCH) treatment, when compared to normal plants. These indicated that RCH treatment could improve the cold-hardiness of . We then investigated the levels of cryoprotectants and metabolic changes in . We found that fed on RCH-treated had higher levels of total sugar, trehalose, proline, glycerol, lipid, lower water content, lower super-cooling point, and increased cold tolerance compared to fed on normal . This suggested that fed on cold-hardened could increase its cold tolerance. Results showed a trophic transmission in insect cold tolerance. Our study enriches the theoretical basis for the co-evolution of cold tolerance in invasive and herbivorous insects.
低温对新引入物种有很大影响，而增强的耐寒性有助于它们在新环境中立足。这种外来入侵物种分布在高纬度和高海拔地区，相比低纬度或低海拔地区，它遭受的冷胁迫更多。冷胁迫是否会影响该物种体内抗冻剂的积累及其耐寒性，进而通过取食影响其生物防治剂的耐寒性，目前尚不清楚。我们研究了该物种体内抗冻剂的水平和代谢变化。我们发现，与正常植株相比，经过快速冷驯化（RCH）处理后，该物种体内总糖、海藻糖、脯氨酸及其他与抗寒相关的代谢物水平有所增加。这些表明RCH处理可以提高该物种的抗寒性。然后我们研究了其生物防治剂体内抗冻剂的水平和代谢变化。我们发现，取食经过RCH处理的该物种的生物防治剂，与取食正常该物种的相比，其总糖、海藻糖、脯氨酸、甘油、脂质水平更高，含水量更低，过冷却点更低，耐寒性增强。这表明取食经过冷驯化的该物种的生物防治剂可以提高其耐寒性。结果显示了昆虫耐寒性的营养传递。我们的研究丰富了入侵昆虫和植食性昆虫耐寒性协同进化的理论基础。
