Zhao Xiaowen, Jiang Yuke, Liu Qi, Yang Huichun, Wang Ziting, Zhang Muqing
Guangxi Key Laboratory of Sugarcane Biology, Nanning, China.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning, China.
Front Microbiol. 2020 May 5;11:704. doi: 10.3389/fmicb.2020.00704. eCollection 2020.
Drought is a major abiotic stress affecting plant growth and development. Sugarcane, a sugar crop planted in warmer climate, suffers dramatically from drought stress. Bacterial communities colonizing the rhizosphere, where roots sense drought stress initially, have been well studied for their beneficial effects on plant growth and health. The gene cloned from the sugarcane, , belongs to the DREB2 subgroup of the DREB gene family, which is involved in drought response regulation. Here, we present a detailed characterization of the rhizoplane, rhizosphere, and bulk soil bacterial communities determined using a high-sequencing approach with the transgenic (TG) sugarcane variety GN18 harboring the drought-tolerant gene and its isogenic wild-type (WT) variety FN95-1702 under the same environmental conditions. In addition, the total carbon (TC), total nitrogen (TN), and total phosphorus (TP) contents in each soil area were compared to explore the relationship between bacterial alteration in the TG and WT plants and environmental factors (TC, TN, TP, C:N, C:P, and N:P). Our results showed that the bacterial communities in the rhizosphere and rhizoplane of TG sugarcane were more similar and perfectly correlated with the environmental factors than those of the WT. This suggested that the bacterial communities of the TG plants were altered in response to the changes in root exudates. The results of our study suggest that the change in soil environment caused by transgenic sugarcane alters soil bacterial communities.
干旱是影响植物生长发育的主要非生物胁迫。甘蔗是一种种植于温暖气候地区的糖料作物，深受干旱胁迫之苦。定殖于根际（根系最初感知干旱胁迫的部位）的细菌群落，因其对植物生长和健康的有益作用而得到了充分研究。从甘蔗中克隆的 基因属于DREB基因家族的DREB2亚组，参与干旱响应调控。在此，我们详细描述了在相同环境条件下，利用高通量测序方法对携带耐旱 基因的转基因（TG）甘蔗品种GN18及其同基因野生型（WT）品种FN95 - 1702的根表、根际和土体土壤细菌群落进行的分析。此外，还比较了各土壤区域的总碳（TC）、总氮（TN）和总磷（TP）含量，以探究TG和WT植株中细菌变化与环境因子（TC、TN、TP、C:N、C:P和N:P）之间的关系。我们的结果表明，与WT相比，TG甘蔗根际和根表的细菌群落更为相似，且与环境因子具有完美的相关性。这表明TG植株的细菌群落因根际分泌物的变化而发生了改变。我们的研究结果表明，转基因甘蔗引起的土壤环境变化改变了土壤细菌群落。
