Li Peng, Ye Shuifeng, Liu Hua, Pan Aihu, Ming Feng, Tang Xueming
The Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China.
State Key Laboratory of Genetic Engineering, Institute of Genetics-Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, China.
Front Microbiol. 2018 Jun 29;9:1390. doi: 10.3389/fmicb.2018.01390. eCollection 2018.
The impacts of rice varieties with stacked drought tolerance and insect resistance on soil microbiomes are poorly understood. Hence, the objective of this study was to investigate the effects resulting from the cultivation of the drought-tolerant and insect-resistant rice cultivar, Hanhui3T, on soil physical-chemical properties, and bacterial and fungal community composition. Soil samples of Hanhui3T and conventional rice varieties (Hanhui3 and Zhonghua11) were collected in triplicate at the booting stage, and bacterial and fungal population sizes and community structures were assessed using qPCR and Illumina MiSeq sequencing, respectively. The Bt protein concentration of Hanhui3T was significantly higher than that of Hanhui3 and Zhonghua11, while the pH of Hanhui3T was significantly lower. Bacterial population sizes and community composition were significantly different between Hanhui3T and Hanhui3 (or Zhonghua11), while no similar effects were observed for fungal communities. These differences suggest that the effect of Hanhui3T cultivation on bacterial community composition is stronger than the effect on fungal communities. Moreover, bacterial abundance was positively correlated to soil pH, while bacterial community structure variations were mainly driven by soil pH and Bt protein concentration differences. In conclusion, the abundances and structure of bacterial communities were altered in rhizosphere with Hanhui3T cultivation that changed soil pH and Bt protein concentrations, while fungal communities displayed no such responsiveness.
对具有耐旱和抗虫特性的水稻品种对土壤微生物群落的影响了解甚少。因此，本研究的目的是调查种植耐旱抗虫水稻品种汉恢3T对土壤理化性质以及细菌和真菌群落组成的影响。在孕穗期采集汉恢3T和常规水稻品种（汉恢3和中华11）的土壤样本，重复采集三次，分别使用定量聚合酶链反应（qPCR）和Illumina MiSeq测序评估细菌和真菌的种群数量及群落结构。汉恢3T的Bt蛋白浓度显著高于汉恢3和中华11，而汉恢3T的pH值显著更低。汉恢3T与汉恢3（或中华11）之间的细菌种群数量和群落组成存在显著差异，而真菌群落未观察到类似影响。这些差异表明，种植汉恢3T对细菌群落组成的影响强于对真菌群落的影响。此外，细菌丰度与土壤pH呈正相关，而细菌群落结构变化主要由土壤pH和Bt蛋白浓度差异驱动。总之，种植汉恢3T改变了根际土壤的pH值和Bt蛋白浓度，从而改变了细菌群落的丰度和结构，而真菌群落则没有这种响应。
