Bruce Spencer A, Schiraldi Nicholas J, Kamath Pauline L, Easterday W Ryan, Turner Wendy C
Department of Biological Sciences University at Albany - State University of New York Albany NY USA.
Department of Information Technology Services University at Albany - State University of New York Albany NY USA.
Evol Appl. 2020 Jan 23;13(5):935-944. doi: 10.1111/eva.12911. eCollection 2020 May.
, the causative agent of anthrax, is a considerable global health threat affecting wildlife, livestock, and the general public. In this study, whole-genome sequence analysis of over 350 isolates was used to establish a new high-resolution global genotyping framework that is both biogeographically informative and compatible with multiple genomic assays. The data presented in this study shed new light on the diverse global dissemination of this species and indicate that many lineages may be uniquely suited to the geographic regions in which they are found. In addition, we demonstrate that plasmid genomic structure for this species is largely consistent with chromosomal population structure, suggesting vertical inheritance in this bacterium has contributed to its evolutionary persistence. This classification methodology is the first based on population genomic structure for this species and has potential use for local and broader institutions seeking to understand both disease outbreak origins and recent introductions. In addition, we provide access to a newly developed genotyping script as well as the full whole-genome sequence analyses output for this study, allowing future studies to rapidly employ and append their data in the context of this global collection. This framework may act as a powerful tool for public health agencies, wildlife disease laboratories, and researchers seeking to utilize and expand this classification scheme for further investigations into evolution.
炭疽杆菌是炭疽病的病原体，对野生动物、家畜和普通公众构成了相当大的全球健康威胁。在本研究中，对350多个分离株进行全基因组序列分析，以建立一个新的高分辨率全球基因分型框架，该框架既具有生物地理学信息，又与多种基因组检测方法兼容。本研究提供的数据为该物种在全球的多样传播提供了新的线索，并表明许多谱系可能特别适应它们所在的地理区域。此外，我们证明该物种的质粒基因组结构在很大程度上与染色体群体结构一致，这表明该细菌的垂直遗传有助于其进化持久性。这种分类方法是基于该物种的群体基因组结构的首次尝试，对于试图了解疾病爆发起源和近期引入情况的地方及更广泛机构具有潜在用途。此外，我们提供了一个新开发的基因分型脚本以及本研究的全基因组序列分析完整输出，使未来的研究能够在这个全球数据集的背景下快速应用并添加他们的数据。这个框架可能成为公共卫生机构、野生动物疾病实验室以及寻求利用和扩展这一分类方案以进一步研究进化的研究人员的有力工具。
