一个偶然的微小结构修饰导致了一种高度针对碳青霉烯酶的荧光探针。
A minor structure modification serendipitously leads to a highly carbapenemase-specific fluorogenic probe.
机构信息
State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Org Biomol Chem. 2020 Jun 7;18(21):4029-4033. doi: 10.1039/d0ob00114g. Epub 2020 May 20.
PMID:32432265
Abstract
Reported herein is a fluorogenic probe for the detection of carbapenemase activity. This reagent features carbapenem as an enzyme recognition motif and a carbon-carbon double bond between carbapenem and the fluorophore, exhibiting high specificity to all carbapenemases, including metallo carbapenemases and serine carbapenemases, over other β-lactamases.
摘要
本文报道了一种用于检测碳青霉烯酶活性的荧光探针。该试剂以碳青霉烯为酶识别基序，碳青霉烯与荧光团之间具有碳-碳双键，对所有碳青霉烯酶（包括金属碳青霉烯酶和丝氨酸碳青霉烯酶）具有高度特异性，优于其他β-内酰胺酶。
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