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本文引用的文献

1
A review of the influence of treatment strategies on antibiotic resistant bacteria and antibiotic resistance genes.治疗策略对抗生素耐药菌和抗生素耐药基因影响的综述。
Chemosphere. 2016 May;150:702-714. doi: 10.1016/j.chemosphere.2015.12.084. Epub 2016 Jan 13.
2
Ligand-Induced Proton Transfer and Low-Barrier Hydrogen Bond Revealed by X-ray Crystallography.X射线晶体学揭示的配体诱导质子转移和低势垒氢键
J Am Chem Soc. 2015 Jul 1;137(25):8086-95. doi: 10.1021/jacs.5b00749. Epub 2015 Jun 22.
3
Natural Variants of the KPC-2 Carbapenemase have Evolved Increased Catalytic Efficiency for Ceftazidime Hydrolysis at the Cost of Enzyme Stability.KPC-2碳青霉烯酶的天然变体以酶稳定性为代价，提高了对头孢他啶水解的催化效率。
PLoS Pathog. 2015 Jun 1;11(6):e1004949. doi: 10.1371/journal.ppat.1004949. eCollection 2015 Jun.
4
Antibacterial properties and atomic resolution X-ray complex crystal structure of a ruthenocene conjugated β-lactam antibiotic.一种二茂钌共轭β-内酰胺抗生素的抗菌特性及原子分辨率X射线复合晶体结构
Chem Commun (Camb). 2015 Apr 11;51(28):6186-9. doi: 10.1039/c5cc00904a.
5
Variants of β-lactamase KPC-2 that are resistant to inhibition by avibactam.对阿维巴坦抑制作用具有抗性的β-内酰胺酶KPC-2变体。
Antimicrob Agents Chemother. 2015 Jul;59(7):3710-7. doi: 10.1128/AAC.04406-14. Epub 2015 Feb 9.
6
Molecular basis for the catalytic specificity of the CTX-M extended-spectrum β-lactamases.CTX-M 型超广谱β-内酰胺酶催化特异性的分子基础
Biochemistry. 2015 Jan 20;54(2):447-57. doi: 10.1021/bi501195g. Epub 2014 Dec 24.
7
Kinetic and structural requirements for carbapenemase activity in GES-type β-lactamases.GES型β-内酰胺酶中碳青霉烯酶活性的动力学和结构要求。
Biochemistry. 2015 Jan 20;54(2):588-97. doi: 10.1021/bi501052t. Epub 2014 Dec 22.
8
Beta-lactam antibiotics induce a lethal malfunctioning of the bacterial cell wall synthesis machinery.β-内酰胺类抗生素会引发细菌细胞壁合成机制的致命性故障。
Cell. 2014 Dec 4;159(6):1300-11. doi: 10.1016/j.cell.2014.11.017.
9
Molecular mechanisms of substrate recognition and specificity of New Delhi metallo-β-lactamase.新德里金属β-内酰胺酶底物识别及特异性的分子机制
Antimicrob Agents Chemother. 2014 Sep;58(9):5372-8. doi: 10.1128/AAC.01977-13. Epub 2014 Jun 30.
10
BLIP-II is a highly potent inhibitor of Klebsiella pneumoniae carbapenemase (KPC-2).BLIP-II 是一种高效的肺炎克雷伯菌碳青霉烯酶（KPC-2）抑制剂。
Antimicrob Agents Chemother. 2013 Jul;57(7):3398-401. doi: 10.1128/AAC.00215-13. Epub 2013 Apr 15.

肺炎克雷伯菌碳青霉烯酶（KPC-2）底物识别与产物释放的分子基础

Molecular Basis of Substrate Recognition and Product Release by the Klebsiella pneumoniae Carbapenemase (KPC-2).

作者信息

Pemberton Orville A, Zhang Xiujun, Chen Yu

机构信息

Department of Molecular Medicine, University of South Florida , 12901 Bruce B. Downs Boulevard, Tampa, Florida 33612, United States.

出版信息

J Med Chem. 2017 Apr 27;60(8):3525-3530. doi: 10.1021/acs.jmedchem.7b00158. Epub 2017 Apr 17.

DOI:10.1021/acs.jmedchem.7b00158
PMID:28388065
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5506774/
Abstract

Carbapenem-resistant Enterobacteriaceae are resistant to most β-lactam antibiotics due to the production of the Klebsiella pneumoniae carbapenemase (KPC-2) class A β-lactamase. Here, we present the first product complex crystal structures of KPC-2 with β-lactam antibiotics containing hydrolyzed cefotaxime and faropenem. They provide experimental insights into substrate recognition by KPC-2 and its unique cephalosporinase/carbapenemase activity. These structures also represent the first product complexes for a wild-type serine β-lactamase, elucidating the product release mechanism of these enzymes in general.

摘要

耐碳青霉烯类肠杆菌科细菌由于产生肺炎克雷伯菌碳青霉烯酶（KPC-2）A类β-内酰胺酶而对大多数β-内酰胺类抗生素耐药。在此，我们展示了KPC-2与含有水解头孢噻肟和法罗培南的β-内酰胺类抗生素的首个产物复合物晶体结构。它们为KPC-2的底物识别及其独特的头孢菌素酶/碳青霉烯酶活性提供了实验见解。这些结构也代表了野生型丝氨酸β-内酰胺酶的首个产物复合物，总体上阐明了这些酶的产物释放机制。