Food Science and Nutrition Department, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia.
Food Science and Nutrition Department, Faculty of Home Economics, Helwan University, Cairo, Egypt.
Biosci Rep. 2020 Jun 26;40(6). doi: 10.1042/BSR20201062.
In the present study, a total of 50 raw camel meat samples were analyzed for the presence of Listeria monocytogenes. The isolates were characterized via morphological and culture analyses; identification of isolates was confirmed by polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The API Listeria system was used for further chemical identification and verification of the strains. L. monocytogenes was identified in eight raw camel meat samples, which was the highest incidence (16%) of contamination, followed by L. seeligeri 3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were positive for the listeriolysin O gene were >99% similar to the published database sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We studied the antibiotic resistance profile of the L. monocytogenes strains with common antibiotics used to treat human listeriosis and demonstrated that almost all strains tested were susceptible to the antibiotics.
在本研究中，分析了 50 份生骆驼肉样本，以检测单核细胞增生李斯特菌的存在。通过形态学和培养分析对分离株进行了特征描述；通过聚合酶链反应 (PCR) 和溶血素 O 基因测序确认了分离株的鉴定。API Listeria 系统用于进一步的化学鉴定和菌株验证。在 8 份生骆驼肉样本中鉴定出单核细胞增生李斯特菌，其污染率最高（16%），其次是 L. seeligeri 3（6%）、L. innocua 和 L. welshimeri 2（各 2%），L. grayi 1（1%）。根据基本局部比对搜索工具 (BLAST) 分析，对溶血素 O 基因呈阳性的分离株与已发表的单核细胞增生李斯特菌菌株 LM850658（序列 ID：CP009242.1）的数据库序列相似性>99%。我们研究了用于治疗人类李斯特菌病的常用抗生素对单核细胞增生李斯特菌菌株的抗生素耐药谱，并表明几乎所有测试的菌株均对这些抗生素敏感。
