Department of Immunology, Nantong University, School of Medicine.
Immunotherapy. 2020 Jun;12(9):653-664. doi: 10.2217/imt-2019-0139. Epub 2020 May 21.
Adoptive cell transfer has attracted considerable attention as a treatment for cancer. The success of chimeric antigen receptor (CAR)-engineered T (CAR-T) cells for the treatment of haematologic tumors has demonstrated the potential of CAR. In this review, we describe the current CAR-engineered natural killer (CAR-NK) cell construction strategies, including the design principles and structural characteristics of the extracellular, transmembrane and intracellular regions of the CAR structure. In addition, we review different cellular carriers used to develop CAR-NK cells, highlighting existing problems and challenges. We further discuss possible ways to optimize CAR from the perspective of the tumor microenvironment to harness the strength of CAR-NK cells and provided rationales to combine CAR-NK cells with other treatment regimens to enhance antitumor effects.
过继细胞转移作为癌症治疗方法引起了广泛关注。嵌合抗原受体 (CAR)-修饰 T 细胞 (CAR-T) 细胞治疗血液肿瘤的成功证明了 CAR 的潜力。在这篇综述中，我们描述了目前的 CAR 修饰自然杀伤 (CAR-NK) 细胞构建策略，包括 CAR 结构的细胞外区、跨膜区和细胞内区的设计原则和结构特征。此外，我们还回顾了用于开发 CAR-NK 细胞的不同细胞载体，强调了存在的问题和挑战。我们进一步从肿瘤微环境的角度讨论了优化 CAR 的可能途径，以发挥 CAR-NK 细胞的优势，并提出了将 CAR-NK 细胞与其他治疗方案相结合以增强抗肿瘤作用的合理依据。
