Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
Genomics. 2020 Sep;112(5):2978-2989. doi: 10.1016/j.ygeno.2020.05.001. Epub 2020 May 11.
Sodium azide is a commonly used cytochrome oxidase inhibitor that leads to translation repression and RNA granule assembly. The global changes in mRNA abundance in response to this stressor are unknown. RGG-motif proteins Scd6 and Sbp1 are translation-repressors and decapping-activators that localize to and affect the assembly of RNA granules in response to sodium azide stress. Transcriptome-wide effects of these proteins remain unknown. To address this, we have sequenced transcriptome of the: a) wild type strain under unstressed and sodium azide stress, b) Δscd6 and Δsbp1 strains under unstressed and sodium azide stress. Transcriptome analysis identified altered abundance of many transcripts belonging to stress-responsive pathways which were further validated by qRT-PCR results. Abundance of several transcripts was altered in Δscd6/Δsbp1 under normal conditions and upon stress. Overall, this study provides critical insights into transcriptome changes in response to sodium azide stress and the role of RGG-motif proteins in these changes.
叠氮钠是一种常用的细胞色素氧化酶抑制剂，可导致翻译抑制和 RNA 颗粒组装。目前尚不清楚这种应激源下 mRNA 丰度的全球变化。RGG 基序蛋白 Scd6 和 Sbp1 是翻译抑制因子和脱帽激活因子，它们定位于 RNA 颗粒并影响其在叠氮钠应激下的组装。这些蛋白质的转录组范围的影响仍然未知。为了解决这个问题，我们已经对以下两种情况进行了转录组测序：a）在未受胁迫和叠氮钠胁迫下的野生型菌株；b）在未受胁迫和叠氮钠胁迫下的Δscd6 和 Δsbp1 菌株。转录组分析鉴定出许多属于应激反应途径的转录本丰度发生了改变，这些结果通过 qRT-PCR 结果进一步得到验证。在正常条件下和应激条件下，Δscd6/Δsbp1 中的几个转录本的丰度发生了改变。总的来说，这项研究为应对叠氮钠胁迫时转录组的变化以及 RGG 基序蛋白在这些变化中的作用提供了重要的见解。
