Siddiqui S A, Craig A
Clin Biochem. 1978 Apr;11(2):73-4. doi: 10.1016/s0009-9120(78)90758-0.
A semi automated procedure for the determination of 3-methoxy-4-hydroxy-mandelic acid (HMMA) in urine has been evaluated. This procedure offers advantages in simplicity, speed and precision over existing manual techniques which utilize spectrophotometric fluorimetric and gas liquid chromatographic methods. The urine is extracted with ethyl acetate from a highly acidic sample, purified by extraction into acetate buffer and the diazo colour reaction developed. All stages of the procedure are carried out using the Brown and semi-automatic extractor equipped with partition tube. The within and between assay coefficient of variation obtained on pooled freeze-dried urine samples was 6.7% and 7.7% respectively.
已对一种用于测定尿中3-甲氧基-4-羟基扁桃酸（HMMA）的半自动方法进行了评估。与现有的利用分光光度法、荧光法和气液色谱法的手工技术相比，该方法在简便性、速度和精密度方面具有优势。尿液从高酸性样品中用乙酸乙酯萃取，通过萃取到乙酸盐缓冲液中进行纯化，并进行重氮显色反应。该方法的所有步骤均使用配备分配管的布朗半自动萃取器进行。对混合冻干尿样获得的批内和批间变异系数分别为6.7%和7.7%。
