Department of Sport and Health Sciences, College of Life and Environmental Sciences, Heavitree Road, University of Exeter, Exeter, UK.
Department of Surgery, University of Texas Medical Branch & Shriners Hospital for Children, Galveston, TX, USA.
Am J Clin Nutr. 2020 Aug 1;112(2):318-333. doi: 10.1093/ajcn/nqaa092.
Mycoprotein is a fungal-derived sustainable protein-rich food source, and its ingestion results in systemic amino acid and leucine concentrations similar to that following milk protein ingestion.
We assessed the mixed skeletal muscle protein synthetic response to the ingestion of a single bolus of mycoprotein compared with a leucine-matched bolus of milk protein, in rested and exercised muscle of resistance-trained young men.
Twenty resistance-trained healthy young males (age: 22 ± 1 y, body mass: 82 ± 2 kg, BMI: 25 ± 1 kg·m-2) took part in a randomized, double-blind, parallel-group study. Participants received primed, continuous infusions of L-[ring-2H5]phenylalanine and ingested either 31 g (26.2 g protein: 2.5 g leucine) milk protein (MILK) or 70 g (31.5 g protein: 2.5 g leucine) mycoprotein (MYCO) following a bout of unilateral resistance-type exercise (contralateral leg acting as resting control). Blood and m. vastus lateralis muscle samples were collected before exercise and protein ingestion, and following a 4-h postprandial period to assess mixed muscle fractional protein synthetic rates (FSRs) and myocellular signaling in response to the protein beverages in resting and exercised muscle.
Mixed muscle FSRs increased following MILK ingestion (from 0.036 ± 0.008 to 0.052 ± 0.006%·h-1 in rested, and 0.035 ± 0.008 to 0.056 ± 0.005%·h-1 in exercised muscle; P <0.01) but to a greater extent following MYCO ingestion (from 0.025 ± 0.006 to 0.057 ± 0.004%·h-1 in rested, and 0.024 ± 0.007 to 0.072 ± 0.005%·h-1 in exercised muscle; P <0.0001) (treatment × time interaction effect; P <0.05). Postprandial FSRs trended to be greater in MYCO compared with MILK (0.065 ± 0.004 compared with 0.054 ± 0.004%·h-1, respectively; P = 0.093) and the postprandial rise in FSRs was greater in MYCO compared with MILK (Delta 0.040 ± 0.006 compared with Delta 0.018 ± 0.005%·h-1, respectively; P <0.01).
The ingestion of a single bolus of mycoprotein stimulates resting and postexercise muscle protein synthesis rates, and to a greater extent than a leucine-matched bolus of milk protein, in resistance-trained young men. This trial was registered at clinicaltrials.gov as 660065600.
真菌来源的蛋白富含 Mycoprotein 是一种可持续的蛋白质食物来源，其摄入会导致全身氨基酸和亮氨酸浓度类似于牛奶蛋白摄入后的浓度。
我们评估了在休息和运动的肌肉中，与牛奶蛋白相比，摄入单一剂量的真菌蛋白对混合骨骼肌蛋白合成的影响，这些肌肉来自于经过阻力训练的年轻男性。
20 名经过阻力训练的健康年轻男性（年龄：22 ± 1 岁，体重：82 ± 2 公斤，BMI：25 ± 1 公斤·m-2）参加了一项随机、双盲、平行组研究。参与者接受了 L-[环-2H5]苯丙氨酸的脉冲输注，并在一次单侧抗阻型运动（对侧腿作为对照）后摄入 31 克（26.2 克蛋白质：2.5 克亮氨酸）牛奶蛋白（MILK）或 70 克（31.5 克蛋白质：2.5 克亮氨酸）真菌蛋白（MYCO）。在运动和蛋白质摄入前以及 4 小时的餐后期间采集血液和股外侧肌样本，以评估静息和运动肌肉中混合肌肉的部分蛋白合成率（FSR）和肌细胞信号对蛋白质饮料的反应。
MILK 摄入后混合肌肉 FSR 增加（从静息时的 0.036 ± 0.008 增加到 0.052 ± 0.006%·h-1，运动时的 0.035 ± 0.008 增加到 0.056 ± 0.005%·h-1；P <0.01），但 MYCO 摄入后增加幅度更大（从静息时的 0.025 ± 0.006 增加到 0.057 ± 0.004%·h-1，运动时的 0.024 ± 0.007 增加到 0.072 ± 0.005%·h-1；P <0.0001）（治疗×时间交互作用效应；P <0.05）。与 MILK 相比，MYCO 后的餐后 FSR 趋势更高（分别为 0.065 ± 0.004%·h-1 和 0.054 ± 0.004%·h-1；P = 0.093），与 MILK 相比，MYCO 后的餐后 FSR 增加幅度更大（分别为 0.040 ± 0.006%·h-1 和 0.018 ± 0.005%·h-1；P <0.01）。
在经过阻力训练的年轻男性中，摄入单一剂量的真菌蛋白可刺激静息和运动后肌肉的蛋白质合成率，其效果比牛奶蛋白中的亮氨酸匹配剂量更明显。这项试验在 clinicaltrials.gov 上注册为 660065600。
