Arboleya Silvia, Saturio Silvia, Suárez Marta, Fernández Nuria, Mancabelli Leonardo, de Los Reyes-Gavilán Clara G, Ventura Marco, Solís Gonzalo, Gueimonde Miguel
Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain.
Diet, Human Microbiota and Health Group, Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Asturias, Spain.
Microorganisms. 2020 May 19;8(5):760. doi: 10.3390/microorganisms8050760.
Correct establishment of the gut microbiome is compromised in premature babies, with being one of the most affected genera. Prematurity often entails the inability to successfully breastfeed, therefore requiring the implementation of other feeding modes; breast milk expression from a donor mother is the recommended option when their own mother's milk is not available. Some studies showed different gut microbial profiles in premature infants fed with breast milk and donor human milk, however, it is not known how this affects the species composition of the genus . The objective of this study was to assess the effect of donated human milk on shaping the gut bifidobacterial populations of premature babies during the first three months of life. We analyzed the gut bifidobacterial communities of 42 premature babies fed with human donor milk or own-mother milk by the 16S rRNA-23S rRNA internal transcriber spaces (ITS) region sequencing and q-PCR. Moreover, metabolic activity was assessed by gas chromatography. We observed a specific bifidobacterial profile based on feeding type, with higher bifidobacterial diversity in the human donor milk group. Differences in specific species composition may contribute to the development of specific new strategies or treatments aimed at mimicking the impact of own-mother milk feeding in neonatal units.
早产儿肠道微生物群的正确建立受到损害，[具体属名]是受影响最严重的属之一。早产往往导致无法成功进行母乳喂养，因此需要采用其他喂养方式；当无法获得母亲自己的母乳时，推荐使用捐赠母乳。一些研究表明，用母乳和捐赠母乳喂养的早产儿肠道微生物特征不同，然而，尚不清楚这如何影响[具体属名]的物种组成。本研究的目的是评估捐赠母乳对早产儿出生后前三个月肠道双歧杆菌种群形成的影响。我们通过16S rRNA - 23S rRNA内部转录间隔区（ITS）测序和q - PCR分析了42名食用捐赠母乳或母亲自己母乳的早产儿的肠道双歧杆菌群落。此外，通过气相色谱法评估代谢活性。我们观察到基于喂养类型的特定双歧杆菌特征，捐赠母乳组的双歧杆菌多样性更高。特定物种组成的差异可能有助于制定特定的新策略或治疗方法，以模拟在新生儿病房中母亲自己母乳喂养的影响。
