Nogacka Alicja M, Arboleya Silvia, Nikpoor Naghmeh, Auger Jeremie, Salazar Nuria, Cuesta Isabel, Alvarez-Buylla Jorge R, Mantecón Laura, Solís Gonzalo, Gueimonde Miguel, Tompkins Thomas A, de Los Reyes-Gavilán Clara G
Department of Microbiology and Biochemistry of Dairy Products, Instituto de Productos Lácteos de Asturias (IPLA-CSIC), 33300 Villaviciosa, Asturias, Spain.
Institute of Health Research of the Principality of Asturias (ISPA), 33011 Oviedo, Asturias, Spain.
Microorganisms. 2022 Jan 29;10(2):318. doi: 10.3390/microorganisms10020318.
2'-fucosyllactose (2'FL) is one of the most abundant oligosaccharides in human milk, with benefits on neonatal health. Previous results point to the inability of the fecal microbiota from some infants to ferment 2'FL. We evaluated a probiotic formulation, including the strains Rosell-52 (R0052), subsp. Rosell-33 (R0033), and Rosell-71 (R0071), individually or in an 80:10:10 combination on the microbiota and 2'FL degradation. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed) with added probiotic formulation or the constituent strains in the presence of 2'FL. Microbiota composition was analyzed by 16S rRNA gene sequencing. Gas accumulation, pH decrease and 2'FL consumption, and levels of different metabolites were determined by chromatography. R0071 was the sole microorganism promoting a partial increase of 2'FL degradation during fermentation in fecal cultures of 2'FL slow-degrading donors. However, major changes in microbiota composition and metabolic activity occurred with R0052 or the probiotic formulation in cultures of slow degraders. Further studies are needed to decipher the role of the host intestinal microbiota in the efficacy of these strains.
2'-岩藻糖基乳糖（2'FL）是母乳中含量最丰富的低聚糖之一，对新生儿健康有益。先前的研究结果表明，一些婴儿的粪便微生物群无法发酵2'FL。我们评估了一种益生菌制剂，包括罗塞尔-52（R0052）、亚种罗塞尔-33（R0033）和罗塞尔-71（R0071）菌株，单独使用或以80:10:10的组合使用，对微生物群和2'FL降解的影响。对来自六名两个月大的足月婴儿供体（三名母乳喂养和三名配方奶喂养）的粪便进行独立批次发酵，在添加益生菌制剂或其组成菌株的情况下，同时添加2'FL。通过16S rRNA基因测序分析微生物群组成。通过色谱法测定气体积累、pH值下降和2'FL消耗以及不同代谢物的水平。在2'FL降解缓慢的供体的粪便培养物中，R0071是唯一能在发酵过程中促进2'FL降解部分增加的微生物。然而，在降解缓慢的培养物中，R0052或益生菌制剂会导致微生物群组成和代谢活性发生重大变化。需要进一步研究来阐明宿主肠道微生物群在这些菌株功效中的作用。
