Boos K S, Wilmers B, Schlimme E, Sauerbrey R
Laboratory for Biological Chemistry, University, Paderborn, F.R.G.
J Chromatogr. 1988 Dec 2;456(1):93-104. doi: 10.1016/0021-9673(86)80009-7.
We developed a coupled dual column system with an optional post-column derivatization for on-line sample processing, trace enrichment and analysis of aromatic 1,2-diol and aliphatic cis-diol biomolecules (e.g. catecholamines, ribonucleosides). The fully automated high-performance liquid chromatography analyzer tolerates the direct injection of proteinaceous fluids by use of a unique bonded-phase precolumn material which allows the simultaneous performance of covalent affinity and size-exclusion chromatography.
我们开发了一种耦合双柱系统，该系统带有一个可选的柱后衍生装置，用于在线样品处理、痕量富集以及分析芳香族1,2 -二醇和脂肪族顺式二醇生物分子（如儿茶酚胺、核糖核苷）。这种全自动高效液相色谱分析仪通过使用一种独特的键合相预柱材料，能够耐受直接进样含蛋白质的流体，该材料允许同时进行共价亲和色谱和尺寸排阻色谱。
